摘要
对 2种测定α-淀粉酶的方法进行比较,结果表明 2-氯- 4-硝基苯酚-α-麦芽三糖苷 (CNP- G3)法与用亚乙基封闭的对硝基苯酚-α-麦芽七糖苷 (EPS或 PNP- G7)法相关性良好, CNP- G3法的优点为直接、一步测定α-淀粉酶活性,不需要辅助酶,试剂稳定, CNP- G3的用量少,价格低廉,是目前较为理想的测定α-淀粉酶的方法。
Two assays,CNP- G3 method and PNP- G7method,forα- amylase were compared. The results suggested that the former was better than the latter. There was a good relativity between both methods but CNP- G3 was more stable, cheaper and without need of other enzymes such asα andβ- glucosidase. It is now more perfect method with one step assay.
出处
《天津药学》
2001年第1期51-52,共2页
Tianjin Pharmacy