虎纹凤梨的组培快繁技术

Tissue Culture and Fast Propagation Techniques of Vriesea splendens

用虎纹凤梨母株两侧的蘖芽经消毒处理后接种到MS＋6-BA 1.0 mg/L＋IAA 0.3 mg/L的培养基中，诱导出愈伤组织。而后用改良MS培养基＋6-BA 0.3 mg/L＋NAA 0.01 mg/L从愈伤中诱导出小芽并继代培养，扩繁。增殖率3.0～4.0倍。续代3～5代后从芽团上选取H＞2.5 cm的粗壮单株仍用改良MS培养基＋6-BA 0.3 mg/L＋NAA 0.01 mg/L扩繁，增殖倍数2～3倍。而芽团上H＜2.5 cm的丛芽分成具2～3个芽的小芽团用改良MS培养基＋6-BA 0.05 mg/L＋IAA 0.01 mg/L＋C 100 mg/L使其长高长粗壮，增殖倍数1.5倍，经过一段时间的培养后切取达到生根标准的单株接到1/4 MS＋IAA 0.3 mg/L＋NAA 0.05 mg/L＋C 500 mg/L培养基中，30～40 d生根率达95%以上。炼苗20 d左右移栽，成活率达85%～90%以上。

The callus could be inducted in MS media added 1.0 mg/L 6-BA and 0.3 mg/L IAA, with sterilized and the tillering buds on both sides of the mother plant of Vriesea splendens. And the small buds could be inducted in modified MS media added 0.3 mg/L 6-BA and 0.01 mg/L NAA from the callus. When the multiply rate （MR value） was up to 3.0~4.0 times, the process would be repeated, and so did it about 3~5 times. Then, the thick single buds more than 2.5 cm high in the bud group were cultured in modified MS media added 0.3 mg/L 6-BA and 0.01 mg/L NAA, the multiply rate （MR value） was up to 2.0~3.0 times. But the buds below 2.5 cm high in the bud group were divided into 2~3 buds-ball cultured in modified MS media added 0.05 mg/L 6-BA, 0.01 mg/L IAA and 100 mg/L active carbon in order to make it grow long and thick, the multiply rate （MR value） was up to 1.5 times. After a period of training, cut per plant which reached the standard root cultured in 1/4 MS media added 0.3 mg/L IAA, 0.05 mg/L NAA and 500 mg/L active carbon, the rooting rate could get to 95% after 30~40 days. The survival rate were more than 85%~90% after the seedlings trained for 20 days were transplanted.