高效液相色谱法测定白花蛇舌草中异高山黄芩素的含量

Determination of isoscutellarein in Hedyotis diffusa willd by HPLC

目的：建立HPLC法测定白花蛇舌草中新活性成分异高山黄芩素含量的方法。方法：采用KromasiC18（150mm×4．6mm，5μm）色谱柱，流动相为乙腈-0．1％磷酸（45：55），检测波长280nm，流速1．0ml·min，柱温30℃。结果：异高山黄芩素在13．2～145．2μg·ml^-1范围内线性关系良好（r=0．9999）。异高山黄芩素的平均样回收率N99．77％（RSD=0．69％）。结论：该方法简便，准确，可靠，可用于白花蛇舌草中异高山黄芩素的含量测定。

OBJECTIVE To develop a method of HPLC for the content determination of isoscutellarein in Hedyotis diffusa willd. METHODS The column was Kromasil C18 （ 150 mm x 4. 6 mm, 5 μm）, mobile phase was acetonitrile-0. 1 G phosphoric acid（45 ： 55）,UV detection wavelength was set at 280 nm, the detector drift tube temperature was set at 30 ℃. RESULTS The calibration curves were linear in the range of 13.2 - 145.2μg· ml^-1 for isoscutellarein（r = 0. 999 9）. The average recovery of isoscutellarein was 99.77 G, （RSD = 0.69%）. CONCLUSION The method is simple, accurate, replicahle, and can be used for the quality control of isoscutellarein in Hedyotis diffusa willd.