摘要
目的：采用高效液相色谱法测定风湿止痛药酒（FSZTYJ）中粉防己碱、防己诺林碱、蛇床子素和二氢欧山芹醇当归酸酯的含量。方法：HypersilC18色谱柱（4．6Fmm×150mm，5μm）；流速：1．0ml·min^-1；粉防己碱和防己诺林碱：流动相为甲醇-乙腈-0．3％二乙胺（52：37：11），检测波长：280nm。蛇床子素和二氢欧山芹醇当归酸酯：流动相A为甲醇，流动相B为0．2mol·L^-1硫酸铵溶液，检测波长：322nm。结果：粉防己碱和防己诺林碱分别在0．1026-2．052μg（r=0．9991）和0．0694～1．388ug（r=0．9995）时进样量与峰面积呈良好的线性关系，平均加样回收率分别为97．30％和96．93％，RSD（n=6）分别为1．44％和1．35％；蛇床子素和二氢欧山芹醇当归酸酯分别在0．0572～1．144μg（r=0．9992）和0．0196～0．392μg（r=0．9994）时进样量与峰面积呈良好的线性关系，平均加样回收率分别为98．19％和96．60％，RSD（n=6）分别为1．50％和1．53％。结论：该方法测定结果准确、灵敏、重复性好。
OBJECTIVE To develop an HPLC method for determination of the content of tetrandrine, fangchinoline, osthole and columbianadin in FSZTYJ. METHODS A Hypersil C18 column was used as the chromatographic column,the flow rate was 1.0 ml,min . Tetrandrine and fangchinoline..The mobile phase consisted of methanol-acetonitrile-0. 3% diethylamine（52：37： 11）. The UV detection wavelength was at 280 nm. Osthole and columbianadin： The mobile phase A consisted of methanol, the mobile phase B consisted of 0. 2 mol · L^- 1 ammonium sulfate solution, The UV detection wavelength was set at 322 nrrL liE- SULTS There was a good linear relationship between the concentration of tetrandrine, fangchinoline and peak area value when the concentrations of tetrandrine and fangchinoline were within the range of 0. 102 6- 2. 052μg（r= 0. 999 1）,0. 069 4- 1. 388 μg（r= 0. 999 5）. The average recovery was 97. 30%（RSD= 1. 44%） and 96. 93 % （RSD= 1.35%）. There was a good linear re- lationship between the concentration of osthole, columbianadin and peak area value when the concentrations of osthole and co- lumbianadin were within the range of 0. 057 2- 1. 144/lg（r= 0. 999 5）,0. 019 6- 0. 392μg（r= 0. 999 4）. The average recovery was 98. 19%（RSD= 1.50%） and 96. 60%（RSD= 1.53%）. CONCLUSION The method was accurate,sensitive,good repeat- ability and could be used for the determination oftetrandrine, fangchinoline, osthole and columbianadin in FSZTYJ.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2014年第8期657-660,共4页
Chinese Journal of Hospital Pharmacy
