针刺足阳明经穴对大鼠胃运动及脑肠肽的影响

Effect of Needling the Points of Foot-Yangming Channel on the Gastric Motility,and Brain-gut Peptides in Rat

目的 :探讨针刺足阳明经穴对胃运动的调整作用与脑肠肽的关系。方法 :以乙醇灌胃造成大鼠胃粘膜损伤模型 ,气囊法测量胃运动频率和波幅的变化率 ,采用放免分析法 (RIA)检测大鼠胃窦及延髓内P物质 (SP)、胃动素 (MTL)及胃泌素 (GAS)含量。结果 :模型组胃运动频率和波幅呈抑制状态 ,针刺四白、天枢、足三里穴可促进胃运动恢复 ,与此同时胃窦、延髓内SP、MTL、GAS含量出现相应变化。三穴比较 :四白主要影响胃窦MTL含量 ,天枢主要影响胃窦SP及延髓GAS含量 ,足三里穴对SP、MTL及GAS均有影响。结论 :推测针刺足阳明经对胃运动的调整作用有脑肠肽参与 ,但上述三穴对胃运动的影响所涉及的脑肠肽不完全一致。

Objective:To clarify whether the peptides are the substantial elements linking stomach and Foot yangming Channel.Methods:The model of gastric mucous damage(GMD) was induced with infusing pure alcohol into gastric cavity.The change rate of frequency and amplitude of gastric motility was monitored by the method of 'Gas Balloon'.Content of substance P(SP) and MTL?GAS in antrum and medulla was measured by radioimmunoassay.Results:Inhibit effect of the frequency and amplitude of gastric motility was showed.After needling the points of Sibai,Tiansu,Zusanli,the gastric motility restored to normal.At the same time levels of SP?MTL and GAS in antrum and in medulla varied analogously.The change of MTL in antrum was found in group of Sibai mostly;the change of SP in antrum mostly in group of Tiansu;the change either SP?MTL or GAS was found in group of Zusanli.Conclusion:There exists certain parallel relationship between level of brain gut peptiads in antrum and medulla and gastric motility.It is suggested that SP and MTL may play a certain role in regulating stomach activity by needling the points of Foot yangming Channel.But their involved peptides may be different.