正交试验法优化卷心菜叶中绿原酸提取工艺研究

Study on the Microwave-assisted Extraction of Chlorogenic Acid from Cabbage Leaves

以卷心菜叶为原料,研究采用微波法辅助提取卷心菜叶中绿原酸的提取工艺。实验中以绿原酸的提取率为指标,以乙醇为提取剂,考察乙醇体积分数、料液比、微波温度、微波时间、微波强度等因素对卷心菜叶中绿原酸提取率的影响。通过L16(45)正交优化绿原酸提取工艺,结果显示,最佳工艺条件为:微波温度60℃、微波时间6 min、微波功率300 W、乙醇体积分数60%、料液比1∶20。在此最佳工艺条件下,卷心菜叶中绿原酸的提取率为1.2682%。

Microwave-assisted extracting was used in extracting Chlorogenic acid from cabbage leaves. With ethanol as extracting agent, the ethanol concentration, the ration of sample to solvent, the microwave temperature, the microwave time, the microwave power were studied by taking the rate of extracting Chlorogenic acid as target from cabbage leaves. The extraction process was optimized through L16 （45） orthogonal test. The results showed that optimum conditions were： microwave temperature 60℃, extraction time of 6min, microwave power 300W, ethanol concentration of 60%, solid-liquid ratio of 1 ： 20. Chlorogenic acid yield was 1.268% under the optimum conditions.