丁桂儿脐贴体外透皮特性考察

Investigation of in vitro Transdermal Permeability of Dingguier Umbilical Pastes

目的：研究丁桂儿脐贴的体外透皮特性.方法：采用立式Franz扩散池进行经皮渗透试验.利用HPLC同时测定丁香酚、桂皮醛、胡椒碱的经皮累积透过量,流动相甲醇（A）-水（B）梯度洗脱（0～25 min,50％ ～ 90％ A）,检测波长分别为290,280,343 nm.结果：丁香酚、桂皮醛及胡椒碱的线性范围分别为0.0144 6 ～2.892 5,0.001 1 ～0.226 2,0.002 0 ～0.403 2μg,平均回收率分别为100.5％,98.6％,99.1％,透皮时滞分别为0.07,0.41,2.36 h,透皮速率依次为49.91,2.65,0.40 μg· cm^-2·h^-1,24 h累积透皮量（x±s）分别为（1204.28±103.74）,（63.01±10.95）,（8.82±1.19） μg·cm^-2.结论：丁桂儿脐贴的体外经皮渗透符合零级动力学过程.

Objective：To investigatein vitro transdermal absorption properties of Dingguier umbilical pastes.Method：Vertical Franz diffusing cells and nude mice were adopted for percutaneous penetration test.HPLC was adopted to determine accumulative percutaneous permeation of eugenol,cinnamic aldehyde and piperine in certain time with mobile phase of methanol （A）-water （B） gradient elution （0-25 min,50%-90% A） and detection wavelength of 290,280,343 nm,respectively.Result：Linear ranges of eugenol,cinnamic aldehyde and piperine were 0.0144 6-2.892 5,0.001 1-0.226 2,0.002 0-0.403 2 μg,average recoveries were 100.5%,98.6%,99.1%,transdermal time-lags were 0.07,0.41,2.36 h,penetration rates were 49.91,2.65,0.40 μg ·cm^-2·h^-1,accumulative penetration amounts in 24 h were （1 204.28 ± 103.74）,（63.01 ±10.95）,（8.82 ± 1.19） μg ·cm^-2,respectively.Conclusion：In vitro percutaneous penetration of Dingguier umbilical pastes complied with zero-order kinetics.