鹿角胶中氨基酸类成分的HPLC指纹图谱

HPLC Fingerprints of Amino Acids Constituents in Cervil Cornus Colla

目的:建立鹿角胶氨基酸类成分的高效液相色谱指纹图谱。方法:采用异硫氰酸苯酯(PITC)柱前衍生法建立高效液相指纹图谱,梯度洗脱进行分离,选用Diamonsil C18(2)(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-醋酸钠缓冲液(pH 6.5),检测波长254 nm,柱温43℃,流速1.0 mL·min-1。结果:共指认13个共有峰,10批样品具有较高的相似度,其液相色谱指纹图谱可反映鹿角胶所含的18种氨基酸成分。结论:该方法稳定可靠,可为鹿角胶中氨基酸成分质量的全面控制提供依据。

Objective：To establish a method of fingerprint analysis of the amino acids on Cervil Comus Colla by HPLC.Method：Cervil Cornus Colla was derivated by Phenyl isothiocyanate （PITC）.The Chromatographic fingerprints were obtained by the Diamonsil C18 （2） column （4.6 mm ×250 mm,5 μm） with the gradient elution solvent system composed of acetonitrie-natrium aceticum buffer solution （pH 6.5）.The detective wavelength was set at 254 nm,the flow rate was 1 mL ·min^-1 and the column temperature was maintained at 43 ℃.Result：The result showed that the amino acids constituents had a high similarity.18 kinds of amino acids in Cervil Comus Colla were detected,of which 13 peaks were identified in common peaks.Conclusion：The method has good reproducibility and stability,and could be used for the research and the quality control of Cervil Cornus Colla.