摘要
目的：建立藏药十味诃子丸的质量控制方法.方法：采用显微鉴别和薄层色谱法进行定性鉴别,采用HPLC法测定羟基红花黄色素A和没食子酸的含量.结果：通过显微可鉴别出红花和诃子;TLC法可鉴别出诃子、红花、山矾叶、紫草茸、獐牙菜和藏茜草及其活性成分的特征斑点;羟基红花黄色素A回归方程为Y=2.526 3 ×10^6X-1.1957 ×10^4（r=0.999 7）线性范围0.013 6 ～0.271 7μg;没食子酸方程Y=3.052 9×10^6X-9.392×10^3（r =0.999 7）,线性范围0.157 9 ～0.947 5μg;平均回收率分别为99.9％,99.1％;RSD分别为2.2％,1.6％（n=6）.结论：建立的方法可全面控制十味诃子丸的质量,鉴别方法专属性强,定量方法准确,可有效控制该制剂的质量.
Objective：To establish a method for quality control of Tibetan Medicine Shiwei Hezi Pills.Method：Traditional Tibetan medicine in prescription were identified by a series of Microstructure and TLC methods.Hydroxysafflor yellow A; gallic acid was determined by HPLC.The separation was performed on Phenomenex C18 column （4.6 mm × 250 mm,5 μm）.Result：The linear range of Hdroxysafflor yellow A and gallic acid was within the range of 0.013 6-0.271 7 μg and 0.157 9-0.947 5 μg.The average recovery was 99.9％ and 99.1,the RSD was 2.2％.and 1.6％.Conclusion：The method can be applied to the quality control of Shiwei Hezi Pills.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第9期51-55,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家药品标准提高项目(民族药414)
关键词
十味诃子丸
红花
诃子
紫草茸
藏茜草
獐牙菜
山矾叶
羟基红花黄色素A
没食子酸
Shiwei Hezi pills
Carthami Flos
Chebulae Fructus
Lacc
Radix et Rhizome Rubiae
Zhangyacai
Folium sympiocos
hydroxysafflor yellow A
gallic acid
