栽培藤茶中功能性成分动态变化

Dynamics Changes of Functional Ingredients from Cultivated Ampelopsis Grossedentata

目的:考察不同采摘时期的两年生栽培藤茶中几种功能性成分多糖、总黄酮、二氢杨梅素和杨梅素的含量动态变化。方法:以蒸馏水为溶剂恒温(90℃)回流提取栽培藤茶中的功能性成分;以苯酚-硫酸比色法、三氯化铝比色法分别在紫外-可见分光光度仪490,318 nm下测定不同采摘时期的两年生栽培藤茶中多糖、总黄酮的含量;采用Dikma ODS C18色谱柱,以甲醇/乙腈(5∶11)-0.1%磷酸水溶液(80∶20)为流动相,流速1 mL·min-1,在HPLC色谱仪紫外检测波长为292,370 nm处分别对二氢杨梅素和杨梅素进行含量测定,柱温为40℃。结果:不同采摘时期的两年生栽培藤茶中多糖、总黄酮、二氢杨梅素和杨梅素的含量差别比较明显,其中多糖以8,9月份含量最高,分别为2.46%和2.41%;总黄酮、二氢杨梅素和杨梅素的含量均是4月份达到最高,分别为8.74%,4.69%,0.18%。结论:本实验所建立的测定不同采摘时期栽培藤茶中4种功能性成分含量变化的分析方法可行,结果可靠,能明确栽培藤茶中这4种功能性成分含量达到最高的最佳采收时期。

Objective：To study the dynamics changes of the content of functional ingredients such as polysaccharide,total flavonoids,dihydromyricetin and myricetin from biennial cultivation of Ampelopsis grossedentata collected in different period.Method：The functional ingredients was rufluxing extracted by distilled water,the extraction temperature is 90 ℃.The contents of polysaccharide and total flavonoids are measured by UV spectrophotometer using phenol-sulfuric acid method （490 nm） and AlCl3 method （318 nm） respectively.The contents of dihydromyricetin and myricetin are measured by HPLC,detected by Dikma ODS C18column （4.6 mm × 250 mm,5 μm） under 40 ℃,the mobile phase was methanol and acetonitrile （5∶11）-0.1% phosphoric acid solution （80∶ 20）,the flow rate was 1 mL ·min^-1,detection wavelengths was 292 nm and 370 nm.Result：The content of polysaccharide,total flavonoids,dihydromyricetin and myricetin of biennial cultivation of Ampelopsis grossedentata of different harvest period had significant differences.The highest content of polysaccharide was in August and September,respectively 2.44% and 2.42% ; the highest content off total flavonoids,dihydromyricetin and myricetin were in April,respectively 8.74%,4.69%,0.18%.Conclusion：The determination method established of this experiment is feasible,reliable,and can make it clear when the best harvest time was of cultural Ampelopsis grossedentata.