参茯益气安神合剂中黄芪甲苷的含量测定

Determination of Astragaloside Ⅳ in Shenfu Yiqi Anshen Mixture by HPLC-ELSD

目的：建立HPLC-ELSD法测定参茯益气安神合剂中黄芪甲苷的含量方法.方法：采用Appllo C18柱（4.6 mm×250 mm,5μm）;流动相乙腈-水（36∶ 64）,流速1.0 mL·min-1,柱温30℃,ELSD参数为漂移管温度80℃,气体流速2.0 L·min-1.结果：黄芪甲苷在2.032 ～12.192μg峰面积积分值自然对数与进样量自然对数有良好的线性关系（r=0.999 9）,平均加样回收率为98.42％,RSD 1.36％.结论：该法操作简单、结果准确,重复性好,可用于参茯益气合剂的质量控制.

Objective：To establish a HPLC-ELSD method for the determination of astragaloside Ⅳ in Shenfu Yiqi Anshen Mixture.Method：HPLC-ELSD was used to determine astragloside Ⅳ in Shenfuyiqi Anshen Mixture,The separation was performed on Appllo C18 column （4.6 mm ×250 mm,5 μm） with the mixture of acetonitrile-water （36∶ 64） as the mobile phase.The flow rate was 1.0 mL ·min^-1,and colume temperature at 30 ℃.The temperature of drift tube was 80 ℃ and zhe carrier flow was 2 L ·min^-1.Resuts：The linear ranges of astragaloside Ⅳ was 2.032-12.192 μg （r =0.9999）,with the average recovery rate were of 98.42％,RSD was 1.36％.Conclusion：The methods is simple,accurate,reproducible and it can used for the quality control of Shenfu Yiqi Anshen mixture.