乌蕨乙酸乙酯部位化学成分

Chemical Constituents of Ethyl Acetate Extract from Stenoloma chusanum

目的:研究乌蕨乙酸乙酯部位的化学成分。方法:利用硅胶柱色谱及Sephadex LH-20柱色谱对乌蕨乙酸乙酯部位进行分离、纯化。通过理化性质和波谱数据对化合物进行结构解析。结果:从乌蕨的乙酸乙酯部位分离、纯化得到13个化合物,分别鉴定为:β-谷甾醇(1),4-羟基-2,6-二氧甲基-苯甲酸(2),胡萝卜苷(3),芹菜素(4),芹菜素-7-O-β-D-吡喃葡萄糖苷(5),4-羟基-3-甲氧基苯甲酸(6),丁香酸(7),原儿茶醛(8),2,5-二羟基苯甲酸(9),原儿茶酸(10),秦皮素(11),6-氯-芹菜素7-O-β-D-吡喃葡萄糖苷(12),牡荆素(13)。结论:化合物6,12为首次从该属中分离得到。

Objective：To study the chemical constituents of ethyl acetate extracts from Stenoloma chusanum.Method：Use silica gel column chromato-graphy and Sephadex LH-20 column chromatography to separate and purify the compounds.Structure identification was carried out by nuclear magnetic resonance （NMR） technology.Result：Thirteen compounds were separated and purified from ethyl acetate parts of S.chusanum,and identified as β-sitosterol （1）,4-hydr-oxy-2,6-dimethoxy-benzoic acid （2）,daucosterol （3）,apigenin （4）,apigenin-7-O-β-D-glucopyranosid （5）,4-hydroxy-3-methoxy-benzoic acid （6）,syringic acid （7）,protocatechuic aldehyde （8）,2,5-dihydroxy-benzoic acid （9）,protocatechuic acid （10）,fraxetin （11）,6-chlorine-apigenin-7-O-β-D-glucopyranosid （12）,vitexin （13）.Conclusion：Compound 6 and 12 are discovered from Stenoloma for the first time.