不同生长期栽培及野生平贝母的总生物碱分布

Alkaloid distribution of Cultivated and Wild Fritillaria ussuriensis from Different Growing Period

目的:研究药用植物平贝母中总生物碱的分布,为平贝母地上部分的开发提供依据。方法:平贝母生物碱组分的分离对比采用薄层色谱法;总生物碱含量测定采用酸性染料比色法。结果:平贝母不同生长部位的生物碱组分存在差别。平贝母栽培品地上部位在营养生长期、花期、果期总生物碱含量分别为地下部位的2.361,2.310,2.193倍,平贝母野生品地上部位在营养生长期、花期总生物碱含量分别为地下部位的1.870,1.496倍。结论:平贝母植株中总生物碱含量虽略有波动,且不同生长部位生物碱组分存在差别,但总体上平贝母地上茎叶的总生物碱含量高于地下鳞茎,存在进一步研究与开发的价值。

Objective：To explore the distribution of the total alkaloid in the Fritillaria ussuriensis,to provide the data basis for the ground part of the F.ussuriensis development and utilization.Method：Thin-layer chromatography was used to preliminary determine whether the alkaloid components in F.ussuriensis different growth area were the same,and total alkaloid content of different origin and growth period of Fritillaria ussuriensis aboveground and underground part by acid dye colorimetry.Result：Therewas a difference in F.ussuriensis alkaloid component of different growth area.Total alkaloid contents in cultivated F.ussuriensis ground parts of the vegetative growth,flowering,fruiting period were 2.361,2.310,2.193 times more than underground parts.Wild F.ussuriensis aerial parts of vegetative growth,flowering phase were 1.870,1.496 times more than underground parts.Conclusion：In F.ussuriensis plants＇ total alkaloid content with some fluctuations,and different growth parts of the alkaloid component there is a difference,but in general the total alkaloid content of F.ussuriensis ground stems and leaves was significantly higher than the underground bulbs,so there is a certain development and utilization value.