胃痞消对胃癌前病变大鼠胃黏膜上皮细胞NF-κBp65表达的影响

Effects of Weipixiao on Expression of NF-κB p65 in Gastric Mucosal Epithelial Cells in Rats with Gastric Precancerous Lesions

目的:探讨健脾化瘀解毒复方胃痞消对胃癌前病变(gastric precancerous lesions,GPL)的疗效及其分子学机制。方法:将SD大鼠随机分为正常组,模型组,维酶素组(0.2 g·kg-1·d-1),胃痞消高、中、低剂量组(15,7.5,3.75 g·kg-1·d-1)。除正常对照组外,其他组均采用N-甲基-N'-硝基-N-亚硝基胍(MNNG)饮水液自由饮用、饥饱失常、耗气泻下法复制GPL大鼠模型,连续18周。从第9周起,给药组按上述剂量连续灌胃给药共10周,观察胃痞消对胃黏膜上皮细胞核因子(NF-κB)p65阳性表达评分的影响。结果:模型组胃黏膜上皮细胞NF-κB p65的表达评分较正常组显著升高(P<0.01);胃痞消各剂量组可不同程度降低NF-κB p65的表达评分(P<0.05),并以胃痞消低剂量组最为显著(P<0.05)。结论:胃痞消能抑制NF-κB信号通路的异常激活,下调NF-κB p65蛋白的表达,能在一定程度上抑制异形细胞的增殖、侵袭、迁移,具有防治胃癌前病变的作用。

Objective：To study the effects of Weipixiao,which has functions of fortifying the spleen,dissipating blood stasis,and detoxifying,on gastric precancerous lesions （GPL） and the related molecular mechanisms.Method：SD rats were randomly divided into normal group,model group,Weimeisu group （0.2 g·kg-1·d^-1）,Weipixiao high dose,middle dose and low dose groups （15,7.5,3.75 g ·kg-1·d^-1）.Except for normal group,the rats in other groups were provided with spontaneous intake of N-methyl-N＇-nitro-N-nitrosoguanidine （MNNG）solution,irregular diet and purgative herbs to establish GPL rat model for 18 consecutive weeks.At the beginning of 9th week,the mediation groups were provided with treatment for l0 weeks at corresponding doses mentioned above,and the expression of nuclear factor-kappa B （NF-κB） p65 in gastric mucosal epithelial cells was observed.Result：Compared with normal group,the scores of NF-κB p65 expression in gastric mucosal epithelial cells of the model group were significantly higher （P 〈 0.01）,but decreased in three different dosage of Weipixiao groups （P 〈 0.05）,particularly in Weipixiao low dose group （P 〈 0.05）.Conclusion：Weipixiao could significantly inhibit the aberrant activation of NF-κB signaling pathway,down-regulating the expression of NF-κB p65 protein.Weipixiao,might probably inhibit the proliferation,invasion and metastasis of atypical cells,as a consequence it can prevent and cure gastric precancerous lesions.