TLR2和MMP2在胎膜早破患者胎盘组织的表达及其意义

Expressions and significances of TLR2 and MMP-2 in placental tissue of patients with premature rupture of membrane

目的:研究Toll样受体2(Toll-like receptor 2,TLR2)和金属基质蛋白酶2(Matrix Metalloproteinase 2,MMP2)在胎膜早破(Premature Rupture of Membrane,PROM)患者胎盘组织中的定位、表达及这两因子之间的关系。方法:选择足月PROM患者30例(实验组)和同孕期正常妊娠妇女30例(对照组),采用免疫组化和RT-PCR法检测实验组和对照组孕妇胎盘组织中TLR2和MMP2的定位与表达。结果:TLR2、MMP2在足月PROM组胎盘组织中的表达比正常晚期妊娠组明显增强,差异有统计学意义(P<0.05);TLR2 mRNA、MMP2 mRNA在足月胎膜早破组胎盘组织中的表达比正常晚期妊娠妇女明显增强,差异有统计学意义(P<0.05)。结论:TLR2和MMP2在胎盘组织的表达可能通过某些机制相互调控,它们的高表达可能与PROM的发生、发展有关。

Objective： To research the locations and expressions of Toll - like receptor 2 （TLR2） and matrix metalloproteinase 2 （MMP- 2 ） in placental tissue of patients with premature rupture of membrane （PROM）, explore the relationship between TLR2 and MMP -2. Methods： Thirty full - term patients with PROM （experimental group） and thirty normal pregnant women （control group） during the same period were selected, immunohistochemical method and RT - PCR were used to detect the locations and expressions of TLR2 and MMP - 2 in placenta tissues of experimental group and normal group. Results ： The expression levels of TLR2 and MMP - 2 in placenta tis- sue of experimental group were statistically significantly higher than those of control group （P 〈 0. 05 ） ; the expression levels of TLR2 mRNA and MMP - 2 mRNA in placenta tissue of experimental group were statistically significantly higher than those of control group （ P 〈 0. 05 ） . Conclusion： TLR2 and MMP - 2 may regulate each other through some mechanisms in placental tissue; the upregulations of TLR2 and MMP -2 may be associated with occurrence and development of PROM.