碘化钠对人甲状腺细胞IL-6、IFN-γ和TNF-α基因表达的影响

Effect of Iodide on Gene Expression of Interleukin-6,Interferon-γ and Tumor Necrosis Factor-α in Cultured Human Thyroid Cells

本研究以 10 4 M碘化钠 (NaI)刺激单层培养的人Graves病 (GD )甲状腺滤泡上皮细胞 (TEC ) ,采用定性及半定量PCR技术检测刺激前后白介素 6 (IL 6 )、γ 干扰素 (IFN γ )和肿瘤坏死因子 α(TNF α )在细胞中的表达水平。结果表明 :(1) 3例GD组织均含有IL 6及IFN γmRNA ,2例检测到TNF α基因表达 ;(2 )基础状态下 ,3例TEC均表达IL 6基因 ,2例表达TNF α ,而所有样本均无IFN γmRNA ;(3)NaI不能诱导TEC产生IFN γmRNA ,对IL 6mRNA的表达亦无明显影响 ;(4 ) 1例TNF α阴性的TEC样本 ,经NaI刺激后 ,可表达mRNA ,2例原含有TNF α的TEC经刺激后 ,其mRNA的表达水平显著增加。提示碘可通过诱导或增强TEC表达TNF α ,导致自身免疫性甲状腺疾病的发生与发展。

To investigate the influence of iodide on cytokine gene expression in thyroid cells and the effect of iodide in the pathogenesis of autoimmune thyroid diseases(AITD) Thymocytes were cultured in the absence or presence of 10 4 M NaI Interleukin 6(IL 6), tumor necrosis factor α(TNF α) and interferon γ(IFN γ) mRNA levels in thyroid cell were detected by semiquantitative reverse transcription polymerase chain reaction(RT PCR) Results showed:(1)IL 6,TNF α and IFN γ were detected in 3/3,2/3 and 0/3 unstimulated cell samples, respectively (2)NaI did not induce gene expression of IFN γ in thyroid epithelial cells(TEC), and could not stimulate IL 6 mRNA expression ,while TNF α mRNA level was significantly increased by NaI It concludes that iodide exerts its important roles in the initiation and progression of AITD through TNF α production