PMA协同IM刺激新生儿脐血淋巴细胞增殖及钙离子浓度的变化

Induction of Proliferative Responses of Neonatal Cord Blood Lymphocytes by Costimulation of PMA and Ionomycin and Change of Intracellular Ca^(2+) Concentration

本文比较新生儿脐带血和成人外周血淋巴细胞对抗CD3单克隆抗体、PMA和IM刺激的增殖反应及细胞内钙离子浓度的变化 ,了解新生儿出生时淋巴细胞功能缺陷可能的环节。结果表明应用抗CD3单克隆抗体或PMA协同IM刺激 ,脐带血淋巴细胞增殖反应及细胞内钙离子浓度明显增强 ,PMA协同IM刺激 ,脐带血淋巴细胞的增殖反应更接近于成人。由于PMA协同IM刺激可部分改善新生儿脐带血淋巴细胞增殖反应的缺陷 ,提示细胞信号传导的上游缺陷可能为新生儿出生时淋巴细胞功能低下的原因之一。

To compare the proliferative responses between the neonatal cord blood lymphocytes and the peripheral blood lymphocytes and to study the changes of intracellular Ca 2+ concentration,stimulated by anti CD3 McAb,PMA and IM,the proliferative responses of lymphocytes were detected by 3 TdR incorporation and the Ca 2+ concentration of lymphocytes were examined by fluorospectrometry The experimental results showed that the prolipherative responses of both cord blood lymphocytes and the adult peripheral blood lymphocytes to anti CD3 antibodies or PMA+IM were significantly increased,but the responses of cord blood lymphocytes to anti CD3 antibodies was lower than those of the adult peripheral blood lymphocytes,and the responses of cord blood lymphocytes to PMA+IM were relatively close to those of the adult lymphocytes The intracellular Ca 2+ concentration also elevated in both cord blood and adult blood lymphocytes Because PMA+IM costimulation can partially improve the proliferative deficiency of neonatal cord blood lymphocytes,it suggests that defects on the signal transduction might be one cause of the neonatal immunodeficiency