横坑切梢小蠹对植物挥发物的电生理和行为反应

Electroantennogram and behavioral responses of Tomicus minor(Hartig)(Coleoptera:Scolytidae) to plant volatiles

为筛选出适宜配制横坑切梢小蠹(Tomicus minor Hartig)引诱剂的有效化合物,测定了横坑切梢小蠹雌、雄成虫对寄主云南松主要挥发性化合物的触角电位(EAG)和行为反应。结果表明:与对照相比,在10 g·L-1浓度刺激下所测试的9种植物挥发物都能引起横坑切梢小蠹雌、雄虫触角的EAG反应,且触角对不同挥发物的EAG反应差异显著(P<0.05),其中雌、雄虫对(+)-α-蒎烯、(+)-柠檬烯、(+)-3-蒈烯、β-蒎烯和异松油烯的EAG反应较强;在剂量反应试验中,雌、雄虫的EAG反应都随着(+)-α-蒎烯、(+)-柠檬烯、(+)-3-蒈烯和β-蒎烯浓度的增加而增强,当浓度增加到10 g·L-1时,EAG反应最强;而雌、雄虫对异松油烯的EAG反应在1 g·L-1最强;除(+)-柠檬烯没有明显的定向作用外,(+)-α-蒎烯、β-蒎烯、(+)-3-蒈烯和异松油烯对横坑切梢小蠹均表现出了明显的引诱作用;行为反应多重比较表明,(+)-α-蒎烯在10 g·L-1浓度下对雌雄虫的引诱作用最大。该研究结果可为开发横坑切梢小蠹林间引诱剂提供参考。

In order to screen out the effective compounds for preparation of attractants to trap fe- male and male Tomicus minor （Hartig）, the electroantennogram （EAG） and behavioral respon- ses of female and male adults of 11. minor to volatiles of its plant host, Pinus yunnanensis Franehet were tested. The results showed that all the 9 tested plant volatiles could induce the EAG responses at 10 g ~ L-1 compared with the control, and the EAG responses of the antennas caused by the different volatiles had significant differences （P〈0.05）. The female and male a- dults of T. minor exhibited strong EAG responses to （＋） -α-pinene, （＋） -limonene, （＋） -3-care- he, β-pinene and terpinolene; In the dose response tests, the EAG responses of female and male adults to （＋）-α-pinene, （＋）-limonene, （＋）-3-carene and β-pinene intensified with the in- creasing concentration. When the concentration increased to 10 g ~ L-1, the EAG response was the strongest; however, the females and males showed the strongest EAG responses to terpinolene at the concentration of 1 g · L-1. Furthermore, T. minor showed no distinct directional behavior to the （＋） -limonene ; however, （＋） -α-pinene, β-pinene, （＋） -3-carene and terpinolene could obviously allure the females and males. The Duncan＇ s multiple range test for the behavioral re- sponse of females and males to the five plant volatiles showed that （＋） -α-pinene had the greatest attracting effect on females and males at 10 g · L-1 concentration. Our study provides a reference for developing attractive chemicals for T. minor in forests.