CdS量子点-高锰酸钾-鲁米诺化学发光体系测定对乙酰氨基酚

CdS QDs-KMnO_4-Luminol Chemiluminescence System for Determination of Acetaminophen

基于对乙酰氨基酚在碱性介质下对CdS量子点-高锰酸钾-鲁米诺化学发光体系强烈的抑制作用,建立了对乙酰氨基酚的流动注射化学发光测定方法。通过正交试验优化的测试条件为：5.0×10-3 mol·L-1 NaOH、1.0×10-5 mol·L-1 KMnO4、6.0×10-4 mol·L-1CdS量子点、5.0×10-4 mol·L-1鲁米诺、样品（标准品或水）的体积比为1∶1∶4∶2∶1;方法对对乙酰氨基酚的线性范围为1.0×10-8~1.0×10-4 mol·L-1,相关系数R为0.9991,检出限为6.0×10-10 mol·L-1;对1.0×10-6 mol·L-1的对乙酰氨基酚溶液平行测定11次,其相对标准偏差（RSD）为0.6%。该方法可应用于药物中对乙酰氨基酚含量的测定。

A sensitive flow injection chemiluminescence （FL-CL） method for the determination of acetaminophen was developed. The method was based on that acetaminophen could inhibit the CL reaction of CdS quantum dots-luminol-potassium permanganate in sodium hydroxide. The test conditions were optimized by orthogonal test. The volume ratio of 5.0 × 10-3 mol. L-1 NaOH, 1. 0 × 10 -5 mol. L-1 KMnO4,6.0 × 10-4 mol .L-1 CdS QDs, 5.0 × 10-4 mol.L-1 luminol and the samples （standard or water） was 1：1：4：2：1. The detection limit of the method was 6. 0×10-10mol.L-1. The linear range for acetaminophen was 1.0× 10-8-1.0× 10-4mol.L-1. The relative standard deviation was 0.6% for 1.0 ×10-6mol . L-1 acetaminophen in 11 repeated measurements. This method could be applied to the determination of acetaminophen in medicament sample.