液相色谱-串联质谱快速测定烟草中18种氨基酸

Fast Analysis of Eighteen Amino Acids in Tobacco Using Liquid Chromatography-Tandem Mass Spectrometry

建立了液相色谱-串联质谱法（LC-MS/MS）快速测定烟草中18种游离氨基酸的分析方法。结果表明,18种氨基酸的检出限（S/N=3）为0.5×10-4~0.1mol/L,线性相关系数均大于0.9990,峰面积测定的相对标准偏差（RSD）为0.42%~8.09%,低、中、高3个添加水平的平均回收率为90.0%~106.0%。该方法准确、灵敏,可用于烟草样品中氨基酸的分析检测。

A method for fast analysis of 18 amino acids in tobacco was developed using liquid chromatography- tandem mass spectrometry（LC-MS/MS）. The results showed that the limits of detection（LODs） of 18 amino acids were in the range of 0.5 × 10 -4-0.1 mol/L（S/N= 3）, the linear correlation coefficients were above 0. 9990 in all cases, and the relative standard deviations（RSDs） of peak areas of the extracted ion chromatograms were in the range of 0.42 %-8.09 %. The average recoveries of the target compounds spiked in the sample at three concentration levels were in the range of 90.0%-106.0%. The developed method is of good efficiency, sensitivity and selectivity, and can be successfully used to determine amino acids in various tobacco samples.