雄激素受体对人乳腺癌细胞侵袭和迁移影响的研究

The effect of androgen receptor on migration and invasion of human breast cancer cells

目的:探讨雄激素受体(Androgen receptor,AR)对人三阴性乳腺癌细胞侵袭和迁移的影响。方法:采用Real-time Q-PCR检测七株人乳腺癌细胞中AR的表达,AR敲低后,通过细胞迁移实验及细胞免疫荧光观察AR敲低对乳腺癌细胞迁移力及细胞表面黏着斑形成的影响;乳癌细胞采用AR阻断剂比卡鲁胺(Bicalutamide)处理后,通过细胞迁移实验、Transwell小室检测Bicalutamide对细胞侵袭和迁移力的影响。结果:AR在三阴性乳癌细胞中高表达;AR敲低能明显降低乳腺癌细胞的迁移力,并且明显抑制细胞表面黏着斑的形成;细胞迁移实验及侵袭实验显示AR阻断剂可显著抑制乳腺癌细胞的迁移和侵袭(P<0.01)。结论:AR shRNA及AR阻断剂能明显抑制人乳腺癌细胞的侵袭、迁移及黏着斑的形成。

Objective To evaluate the influence of androgen receptor （AR） on the migration and invasion of human triple-negative breast cancer cells. Methods Real-time Q-PCR was performed to detect the AR expression in 7 cell line of breast cancer. The effect of AR knockdown on migration and focal adhesion formation of breast cancer cells were assessed by cell migration and immunofluorescence assay. Breast cancer cells was treated with AR antagonist Migration assay and transwell migration assay were performed to detect the migration and invasion of treated cells. Results AR was highly expressed in triple-negative breast cancer cells. AR knockdown dramatically decreased the migration and inhibited large focal adhesion （FA） formation in. Migration and invasion assay showed that AR antagonist also inhibit the migration and invasion of breast cancer cells （P 〈 0.001）. Conclusions AR knockdown and antagonistdramatically inhibit the migration.invasion and FA formation of human triple-negative breast cancer cells.