miR-191靶位点SNP与胃癌发病风险的关系

Association between single nucleotide polymorphism at miR-191 target site and susceptibility to gastric cancer

目的：探讨miR-191靶位点SNP与胃癌易感性的关系。方法：（1）运用生物信息学技术预测并筛选出miR-191靶位点的SNP；（2）采用病例-对照研究，通过PCR—RFLP方法检查所选取的靶基因的SNP。（3）通过非条件Logistic回归分析该位点与胃癌易感性的关系。结果：（1）生物信息学分析选取SHOC2rsl327552，BRD3rs433402，MDM4rs4245739为候选基因研究：（2）对469例胃癌患者和494例正常组的候选基因SNP分型，rsl327552中含有G等位基因的基因型（AG＋GG）患胃癌的风险高AA基因型P〈0．001。OR 1．693，95％CI 1．286—2．228，而rs43340、rs4245739则与患胃癌的风险无统计学意义。进一步分层分析结果发现rsl327552AG＋GG基因型与胃癌患者较晚的临床分期有关P=0．003，OR2．17．95％CI1．07。4．39。结论：SHOC2基因的rsl327552多态性位点与胃癌发病存在显著性关联．G等位基因可能是胃癌发生的-个遗传危险因素。

Objective The study aims to investigate the association between SNP at miR-191 target site and the susceptibility to gastric cancer. Methods Bioinformatics survey was used to explore SNPs within miR- 191 binding sites. A case-control study and genotypes assay using PCR-RFLP method were conducted to explore the relationship between SNPs and gastric cancer. Results Three SNPs （rs1327552, rs433402, rs4245739） of target genes within miR-191 binding sites were selected by using bioinformatics survey and genotyped. The case- control study found that only G allele genotypes （rs1327552 AG and GG） of shoc2 were found to be associated with significantly increased GC risk. After gastric cancer cases were stratified according to age, sex, smoking and drinking or chnic stage, they showed that the frequencies of shoc2 genotypes （rs1327552 AG and GG） were found to be significantly associated with a later stage of GC. Conclusion The polymorphism of rs1327552 in SHOC2 is associated with GC. Thus, the G allele may be an inherited risk factor for GC development.