摘要
目的:探讨T细胞免疫球蛋白粘蛋白-3(TIM-3)在男性前列腺癌组织中表达及意义。方法:通过蛋白印迹法(Western blot)和免疫组化法测定TIM-3蛋白在69例前列腺癌标本及邻近良性组织的表达。同时利用四甲基偶氮唑盐法(MTT法)进行细胞增殖试验及细胞侵袭试验。评估TIM-3 siRNA在两种人类前列腺癌细胞株上基因抑制作用。结果:TIM-3在前列腺癌中表达高于良性组织。TIM-3蛋白在两种前列腺癌细胞株上也有表达,TIM-3基因抑制可以阻止前列腺癌细胞株增殖及侵袭能力。结论:TIM-3在前列腺癌增殖、侵袭过程可能起重要作用,可能是前列腺癌患者的不良预后因素之一。
Objective To demonstrate the expression and significance of TIM-3 in prostate cancer. Methods TIM-3 protein expression was detected by Western blot and immunohistochemistry in tumor tissues and the paired adjacent benign tissues from 69 patients with prostate cancer. Cell proliferation and cell invasion were performed by 3-（4,5-dimethylthiazol-2yl）-2,5-diphenyl-2H tetrazolium bromide （MTY） assay. The effect of small interfering RNA （siRNA）-mediated knockdown of TIM-3 （TIM-3 siRNA） in two human prostate cancer cell lines was evaluated. Results The level of TIM-3 in prostate cancer tissue was higher than that in the benign tissue. TIM-3 protein was also detected in two prostate cancer cell lines. Knockdown of TIM-3 suppressed the capacities of the prostate cancer cell proliferation and invasion. Conclusions TIM-3 playes an important role in the proliferation and invasion of the prostate cancer cell and may a poor prognosis factor of prostate cancer.
出处
《实用医学杂志》
CAS
北大核心
2014年第8期1241-1244,共4页
The Journal of Practical Medicine
基金
延边大学科技发展计划项目(编号:201259)
