干扰素废水菌种的筛选与产酶特性

Screening and Enzymatic Characteristics of Bacteria from Interferon Wastewater

针对干扰素废水具有抑制性导致活性污泥驯化培养难的问题,利用稀释涂布、富集培养的方法从干扰素废水中筛选出5株高效菌株,分别为IFN1~IFN5。观察5株高效菌种的菌落形态,经16S rDNA测序鉴定其中2株菌株为假单胞菌,2株菌株为索氏菌,1株为不动杆菌。完成了5株高效菌种的生理生化实验,分析其酶代谢系统：IFN1可产生蛋白酶和色氨酸酶;IFN2可产生过氧化氢酶、色氨酸酶和蛋白酶;IFN3可产生过氧化氢酶、色氨酸酶、蛋白酶和淀粉水解酶;IFN4可产生过氧化氢酶、蛋白酶和淀粉水解酶;IFN5可产生过氧化氢酶、蛋白酶。本研究为后续针对降解干扰素废水诱导酶的提取奠定基础。

In order to solve the problem of interferon inhibition in the culture of activated sludge bacteria in wastewater, five bacteria strains were screened from interferon wastewater with the method of dilution and enrichment,namely IFN1,IFN2,IFN3,IFN4 and IFN5. The colony morphology of the five strains was observed,and the isolates were identified as two Pseudomonas spp,two Thauera spp,and one Acinetobacter sp. by 16S rDNA sequence identification. The analyses of physiology,biochemistry and enzymatic metabolism of these five bacteria strains showed that protease was excreted by all strains and tryptophanase was excreted by IFN1,IFN2 and IFN3 strains. In addition,catalase was excreted by IFN1,IFN2,IFN4 and IFN5,and amylase was excreted by IFN3 and IFN4 strains. This research will provide foundation for extracting enzymes to degrade interferon wastewater.