心得宁口服液对大鼠慢性心衰模型的影响

Effect of Practolol Oral Liquid on Chronic Heart Failure Rat Model

目的观察心得宁口服液对大鼠慢性心衰模型心脏动力学和血清脑钠肽(BNP)、肌钙蛋白I(cTnI)、乳酸脱氢酶(LDH)水平及左心室指数、全心指数的影响。方法 60只大鼠随机分为5组,分别为模型组、假手术组和高、中、低浓度的心得宁口服液组,并设阳性对照组(灌服芪苈强心胶囊混悬液)。假手术组及模型组均灌服同体积生理盐水,连续给药6周后,测定各组大鼠心脏动力学变化,血清BNP、cTnI、LDH水平及左心室指数、全心指数。结果慢性心衰模型造模成功。与模型组比较,中剂量心得宁口服液组可明显升高心脏HR水平(P<0.05),高、中剂量心得宁口服液组可明显升高心脏LVSP水平(P<0.05),各给药组均有降低心脏LVEDP水平、升高心脏左心室内压变化速率的趋势(P>0.05)。中、低剂量心得宁口服液组可明显降低血清BNP水平(P<0.05);高剂量心得宁口服液组可明显降低血清cTnI水平(P<0.05),各给药组可显著降低血清LDH水平(P<0.01),中剂量心得宁口服液组可显著降低左心室指数、全心指数(P<0.01),低剂量心得宁口服液组可明显降低左心室指数(P<0.05)。结论心得宁口服液具有明显改善大鼠慢性心衰模型的作用。

OBJECTIVE To observe the effect of practolol oral liquid on cardiodynamics, level of BNP, cTnI, LDH in serum, left ventricular index and heart index of chronic heart failure rat model. METHODS Sixty rats were divided into 6 groups randomly： model group, sham-operated group, high, medium, low dose practolol oral liquid groups, and Qiliqiangxin capsule suspension group. Model group and sham-operated group were fed with normal saline. Administered continuously for 6 weeks, cardiodynamics, level of BNP, cTnI, LDH in serum, left ventricular index and heart index were determined. RESULTS Chronic heart failure rat model was established successfully. Compared with model group, the medium dose group could increase cardiac HR levels obviously（P〈0.05）, the high and medium dose groups could increase cardiac LVSP level obviously（P〈0.05）. Every group had the trend of reducing cardiac LVEDP level and increasing cardiac change rate of LVSP（P〉0.05）. The medium and low dose groups could reduce BNP level in serum obviously（P〈0.05）, the high dose group could reduce cTnI level in serum obviously（P〈0.05）, every group could reduce LDH level significantly（P〈0.01）, the medium dose group could reduce left ventricular index and heart index significantly（P〈0.01）, the small dose group could reduce left ventricular index obviously（P〈0.05）. CONCLUSION Practolol oral liquid has the effect of ameliorating chronic heart failure of rat.