头孢噻肟钠与吖黄素的荧光猝灭反应及其分析应用

Fluorescence Quenching Reaction of Cefotaxime Sodium with Acriflavine and Their Analytical Applications

在pH 2.1～4.1的Britton-Robinson （BR）缓冲溶液中,头孢噻肟钠（CFTM）与吖黄素（AF）形成1∶1的离子缔合物,导致吖黄素溶液荧光猝灭.当分别于最大激发和最大发射波长（λex/λem=266 nm/506 nm）进行测量时,荧光猝灭值（△F）与CFTM浓度在一定范围呈良好的线性关系.该方法灵敏度高,测定CFTM的线性范围和检出限分别为0.07 ～5.0 mg/L和0.021 mg/L.考察了体系的荧光光谱特征、适宜的反应条件和共存物质的影响,讨论了离子缔合物的组成.基于离子缔合反应,发展了测定CFTM的高灵敏、简便、快速的新方法,将其用于血清和尿液中CFTM的测定,结果满意.

In Britton - Robinson （BR） buffer solution at pH 2. 1 - 4. 1, acriflavine （AF） reacted with cefotaxime sodium（CFTM） to form an ion association complex that quenched the fluorescence of acriflavine and whose compound ratio was 1 ： 1. The measurement wavelength of fluorescence quenching was located at λex/λem =266 nm/506 nm. The linear range for CFTM ranged from 0. 07 mg,/L to 5.0 rag/L, and its detection limit was 0. 021 mg/L. In this study, the fluorescence spectra characteristics, the optimum reaction conditions and effect of foreign substances were investigated. The composition of ion-association complexes was discussed. Based on the ion-association reaction, a high sensitive, simple and rapid method for the determination of CFFM was proposed. The method was applied for the determination of CFFM in human serum and urine samples with satisfactory re- sults.