2011年莱州市部分健康人群和家养动物新布尼亚病毒抗体调查

Survey on Antibody of New Bunyavirus Amoug Healthy People and Domestic Animals,Laizhou City,2011

目的了解莱州市发热伴血小板减少综合症病毒(简称:新布尼亚病毒,SFTSV)在莱州市健康人群和家养动物中的流行特点,为疫情预防和控制提供依据。方法采用分层随机抽样方法,采集莱州市不同年龄组健康人群血清1 065份,用间接法ELISA检测SFTSV IgG抗体,分析不同年龄组、性别、职业间抗体阳性率的差异;采用随机抽样的方法,采集莱州家养动物(牛、羊、狗、猪、鸡)血清1 752份,用双抗原夹心法ELISA检测血清中SFTSV总抗体,对不同生长环境动物SFTSV抗体阳性率进行比较。结果共检测健康人群血清1 065份,SFTSV IgG抗体阳性率3.29%(35/1 065);5种家养动物血清1 752份,SFTSV总抗体阳性率36.64%(642/1 752),其中牛、羊、狗、猪、鸡血清样本SFTSV总抗体阳性率分别为48.50%、54.00%、24.73%、0.00%和55.17%。健康人群中不同性别和年龄组间抗体阳性率差异均无统计学意义(P>0.05)、不同职业间抗体阳性率差异有统计学意义(P<0.05);不同动物间SFTSV总抗体阳性率差异有统计学意义(P<0.01),散养动物与圈养动物血清SFTSV总抗体阳性率差异有统计学意义(P<0.01)。结论莱州市为SFTSV流行地区,健康人群和家养动物中均存在SFTSV的流行,且对SFTSV普遍易感,从事农业生产的劳动者感染风险较高,应加强自我防范意识。

Objective To understand the infections of new Bunyavirus in domesticated animals and healthy population in Laizhou city, so as to provide the basis for its control and prevention. Methods In serological survey of healthy popula- tion,serum samples were collected by multi-stage stratified random sampling from Laizhou city. new Bunyavirus IgG sta- tus was determined by indirect enzyme-linked immunosorbent assay （ELISA）. Specific prevalence about age, gender and occupation were calculated based on the results of ELISA assay. In serological survey of domesticated animals, serum sam- ples were collected by random sampling, new Bunyavirus antibody status was determined by double antigen sandwich ELISA. Results A total of 1 065 serum samples were collected from healthy people in Laizhou city. The seroprevalence was 3.29% （35/1 065）. And a total of 1 752 serum samples were collected from domesticated animals,the seroprevalence was 36.64% （642/1 752）,SFTSV-specific antibodies were detected in 210 of 433 cattle （48.50%）, 243 of 450 sheep （54.00%） ,45 of 182 dogs （24.73%）,0 of 426 pigs （0.00%）,and 144 of 261 chickens （55.17%）. Among the serum of healthy people, the gender and age had no difference in seroprevalence（ P 〉0.05） ,hut the occupation correlated with se- roprevalence（ P 〈0.05） ,Different feeding methods had significant difference （ P 〈0.05）. Conclusion Laizhou city is an epidemic area of new Bunyavirus, and both healthy people and domestic animals are susceptible to the virus. More atten- tion and personal protection should be considered by those engaged in agricultural production.