摘要
目的通过比较强直性脊柱炎(AS)患者与健康人之间TNF-α的表达差异,探讨其在该病发病机制中的作用。方法采用酶联免疫吸附法(ELISA)测定强直性脊柱炎患者和健康人血浆中TNF-α蛋白含量;荧光定量RTPCR法对外周血单个核细胞中TNF-αmRNA表达量进行测定。结果强直性脊柱炎患者血浆TNF-α含量(26.307±15.737ng/L)高于健康人(11.147±13.868ng/L),P<0.05,差异有统计学意义;强直性脊柱炎患者外周血单个核细胞TNF-αmRNA表达量(0.0024±0.0177)显著高于健康人(0.0014±0.0004),且P<0.05,差异有统计学意义。结论 AS患者TNF-α表达量高于健康人,是强直性脊柱炎发病机制中一种重要细胞因子。
Objective Discuss the role of TNF-αin the pathogenesis of AS by comparing the expression of TNF-αdifferences between ankylosing spondylitis patients and healthy controls.Methods Using enzyme-linked immunosor-bent assay （ELISA）method,the levels of TNF-αplasma were detected in the patients of ankylosing spondylitis and healthy controls.To assay the expression of TNF-αmRNA in the PBMC cells between patients and healthy controls by qRT-PCR.Results The contents of TNF-α in AS patients were 26.307 ± 15.737 ng/L,respectively,and obviously higher compared with healthy people with statistical significance （P 〈0.05）;TNF-αmRNA in AS patients and healthy people were （0.0024±0.0177）and （0.0014±0.0004）,respectively,with significant difference （P 〈0.05）.Conclusion mRNA expression level of TNF-αin PBMC from AS patients was higher than that in healthy people which further proves that TNF-αis an important cytokine in the pathogenesis of AS.
出处
《中国实验诊断学》
2014年第4期589-592,共4页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金资助(NO.81172840)
