摘要
毕赤酵母作为表达外源蛋白的宿主,不仅具有原核生物表达系统的优点,如培养成本低、遗传操作简单、表达效率高等,还可以对表达的外源蛋白进行翻译后修饰加工,如蛋白质折叠、二硫键形成和糖基化等,因而有其独特的优势。在分泌蛋白修饰中,糖基化是一种重要修饰方式,影响蛋白的结构与功能。本文就毕赤酵母对表达的分泌蛋白进行糖基化修饰及糖基人源化改造研究进展进行综述。
Pichia pastoris as the host for the expression of recombinant proteins, has not only the advantages of prokaryotic expression system such as inexpensive in culture, ease of genetical manipulation, high levels of protein expression, but also has post-translational protein processing capabilities like other higher eukaryotes such as protein folding, formation of disulfide bond and glycosylation. Gly-cosylation is an important form of posttranslational modification in secreted proteins and affects the structure and function of these pro-teins. In this review, we discuss protein glycosylation and humanized glycosylation engineering in Pichia pastoris.
出处
《福建畜牧兽医》
2014年第2期20-22,共3页
Fujian Journal of Animal Husbandry and Veterinary medicine
关键词
毕赤酵母
O-糖基化
N-糖基化
糖基人源化
Pichia pastoris
O-glycosylation
N-glycosylation
humanized glycosylation
