液相色谱-串联质谱测定植物性食品中啶虫脒、甲霜灵和哒螨灵残留量

Rapid Determination Method of Organophosphorus Pesticide Residues in Tea

建立了植物性食品中啶虫脒、甲霜灵和哒螨灵残留量的液相色谱-串联质谱（ESI源）检测方法.试样经乙腈提取,用V（乙腈）∶V（甲苯）=3∶1的溶液淋洗的固相萃取柱净化后,用LC-MS-MS测定.采用工作曲线法进行校正基质效应引来的定量的误差.3种农药的方法线性范围：0.2～20.0μg/kg,线性关系良好（r≥0.995）.在2.0～10.0 μg/kg的添加水平范围内的平均回收率均大于80.0％,相对标准偏差为均低于15％,3种农药的最低检出限0.2 μg/kg,定量限为1.0 μg/kg.

Establish the gas chromatographic detection method of nine kinds of organophosphorus pesticide in tea. Samples were separation by chromatographic column in gas chromatography DB-XLB, and determined with flame photometry detector （FPD）. The results show that, nine kinds of organophosphoms can be separated, and have good linear relationship between 0.05 ug/mL to 1.0 ug/mL （r~〉0.999）. And the average recovery was 78.6 %-105 % when the added level was between 0.05 mg/kg to 0.20 mg/kg; the relative standard deviation is between 1.4% to 7.8%. The detection limit of nine kinds oforganophosphorus was 0.005-0.001 mg/kg.