L-苯丙氨醇质量控制研究

Quality Control of L-Phenylalaninol

建立L-苯丙氨醇质量内控方法.采用反相高效液相色谱法,用Phenomenex Gemini C18（4.6×250 mm,5μm）柱,以乙腈-0.02 mol/L磷酸二氢钾溶液（含0.1％三乙胺）（8∶92）为流动相,在206 nm测定.L-苯丙氨醇在0.01～0.23μg/μL范围内线性关系良好（r=0.9999）;回收率为100.30％（RSD=1.60％）.采用比旋光度法控制其光学纯度.

To develop the quality standard for L-phenylalaninol. The content of L-phenylalaninol was determined by reversed-phase high performance liquid chromatography（RP-HPLC）with the column of Phenomenex Gemini Ci8（4.6-250mm, 5 p.m） and the mobile phase of acetonitrile：20 mM potassium dihydrogen phosphateat containing 0.1% triethylamine（8 ： 92） at 206 nm. The linear range of L-phenylalaninol was 0.01-0.23μg/μL （r = 0.9999） and the average recovery of L-phenylalaninol was 100.30 %（RSD = 1.60）. The enantiospecific analysis of L-phenylalaninol was monitored by the power rotation.