期刊文献+

基于普鲁士蓝/聚多巴胺/纳米金纳米粒子构建的电流型免疫传感器

A Label-Free Electrochemical Immunosensor Based on Gold Nanoparticles-Polydopamine-Prussian Blue Nanocomposite for Determination of α-Fetoprotein
下载PDF
导出
摘要 首先制备普鲁士蓝纳米粒子(PB),通过利用多巴胺(DA)自氧化聚合形成聚多巴胺膜(Dopa)的特性,在PB纳米粒子表面包覆成膜,有效增加PB的稳定性,并在氯金酸溶液中原位还原沉积金纳米粒子,利用纳米金固定化甲胎蛋白抗体(anti-AFP),制得灵敏度高和稳定性好的无标记免疫传感器.采用透射电子显微镜(TEM)对纳米粒子进行表征,采用循环伏安法(CV)和交流阻抗法(EIS)考察修饰电极的电化学特性,实验结果表明,PB-Dopa-Au纳米粒子修饰的电极在实验过程中呈现出良好的氧化还原活性,对甲胎蛋白的检测范围为0.02~80 ng/mL检出限为0.01ng/mL. In the paper, a simple and highly sensitive amperometric immunosensor for the determination of alpha-fetoprotein (AFP) based on gold nanoparticles-polydopamine-Prussian Blue (PB-Dopa-Au) nanocomposites was fabricated.Prussian blue (PB) nanoparticle was initially synthesized under ultrasonic condition and it was surface functionalized by one-step oxidative polymerization of dopamine in basic solution at environment friendly condition to obtain the polydopamine (Pdop) modified reduced PB nanoparticles. Then, via in situ deposition, the Au NPs deposited on the polydopamine functionalized PB nanocomposites Finally, antibodies were anchored on the Au NPs.The preparation of the multifunctional nanoparticle (PB-Dopa-Au)) was characterized by TEM and the assembly of the biosensor was characterized with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).The dynamic range of the resulted immunosensor for the detection of AFP is from 0.02 ng/mL to 80.0 ng/mL with a detection limit of 0.02 ng/mL (S/N = 3). Moreover, this biosensor displays good selectivity, stability and reproducibility.
出处 《广东化工》 CAS 2014年第8期119-120,126,共3页 Guangdong Chemical Industry
基金 农药拟除虫菊酯残留的电化学生物传感器研究及应用(21065009)
关键词 普鲁士蓝 纳米金 甲胎蛋白 免疫传感器 prussian blue polydopamine gold nanoparticles
  • 相关文献

参考文献7

  • 1Chen C,Liu D,Wu Z,et al.Sensitive label-free electrochemical immunoassay by electrocatalytic amplification[J].Electrochemistry Communications,2009,11(10):1869-1872.
  • 2郭小群.生物传感器应用与发展[J].广东化工,2012,39(12):9-10. 被引量:3
  • 3Kornkova M A,Karyakina E E,Marken F,et al.Hydrogen Peroxide Detection in Wet Air with a Prussian Blue Based Solid Salt Bridged Three Electrode System[J].Analytical chemistry,2013,85(5):2574-2577.
  • 4Garjonyte R,Malinauskas A.Operational stability of amperometric hydrogen peroxide sensors,based on ferrous and copper hexacyanoferrates[J].Sensors and Actuators B:Chemical,1999,56(1):93-97.
  • 5Wang W,Li R,Tian M,et al.Surface silverized meta-aramid fibers prepared by bio-inspired poly (dopamine) functionalization[J].ACS applied materials&inteffaces,2013,5(6):2062-2069.
  • 6Lee H,Dellatore S M,Miller W M,et al.Mussel-inspired surface chemistry for multifunctional coatings[J].Science,2007,318(5849):426-430.
  • 7Du D,Wang M,Cai J,et al.One-step synthesis of multiwalled carbon nanotubes-gold nanocomposites for fabricating amperometric acetylcholinesterase biosensor[J].Sensors and Actuators B:Chemical,2010,143(2):524-529.

二级参考文献15

  • 1Tombelli S,MasciniM,Soca C. A DNA piezoelectric biosensor assay coup led with a polyerase chain reaction for bacterialtoxicity determination in environmental samples[J].AnalyticaChimicaActa,2000,(01):1-9.
  • 2潘松;王国栋.VHDL实用教程[M]成都:电子科技大学出版社,2003.
  • 3Campanella L,Cubadda F,Sammartino M P. An algal biosensor for the monitoring of water toxicity in estuarine enviraonments[J].Water Research,2001,(01):69-76.
  • 4Lee Hae-Ok,Cheun Byeung Soo,Yoo Jong Su. App lication of a channel biosensor for toxicity measurements in cultured Alexandrium tamarense[J].Journal ofNaturalToxins,2000,(04):341-348.
  • 5林明权.VHDL数字系统控制范例[M]北京:电子工业出版社,2003.
  • 6Leth S,Maltoni S,Simkus R. Engineered bacteria based biosensors for monitoring bioavailable heavy metal[J].Electroanalysis,2002,(01):35-42.
  • 7Yoshida N,McNiven S J,Yoshida A. A compact optical system for multi-determination of biochemical oxygen demand using disposable strips[J].Field Analytical Chemistry and Technology,2001,(05):222-227.
  • 8Nakamura H. Phosphate ion determination in water for drinkingusing biosensors[J].Bunseki Kagaku,2001,(08):581-582.
  • 9陆蓉,王章金,任恕.传感针在医学中的应用及前景[J].中国针灸,1999,19(11):685-687. 被引量:17
  • 10石劲松,李晓男,张继红.纳米材料及其在传感器中的应用[J].新技术新工艺,2001(6):35-36. 被引量:12

共引文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部