Attractin在小鼠睾丸组织的表达

Expression of Attractin in the Mouse Testis

目的：检测 Attractin （Atrn）mRNA和 Atrn蛋白在小鼠睾丸组织中的表达以及 Atrn蛋白在睾丸组织的分布.方法：取10~12周龄雄性BALB/c 小鼠睾丸组织,应用逆转录-聚合酶链反应（reverse transcription-polymerase chain reaction,RT-PCR）和Western blot方法,检测 Atrn mRNA和 Atrn蛋白在成熟雄性小鼠睾丸组织中的表达,用免疫组织化学方法检测 Atrn蛋白在睾丸组织的分布.结果：RT-PCR扩增产物经琼脂糖凝胶电泳,显示为单一、特异扩增条带,与预期产物片断大小一致. Western Blot 检测到 Atrn蛋白,分子量约为160KD.免疫荧光组织化学结果显示 Atrn蛋白在小鼠睾丸组织分布广泛,表达于精原细胞、精母细胞、精子细胞、Sertoli细胞、Leydig细胞和管周肌样细胞,主要表达于胞膜.结论：成熟小鼠睾丸组织表达 Atrn mRNA和 Atrn蛋白.Atrn蛋白定位于间质细胞和生精细胞胞膜.

Objective：To examine the expression of Attractin（Atrn）mRNA and Attractin protein in the mouse testis.And then investigate the distribution of Atrn protein in the mouse testis.Methods：Testis from 10~12weeks old BALB/c mice were used for the study of Atrn expression and distribution by RT-PCR, Western blot and immunofluorescence.Results：The Atrn mRNA and Atrn protein were both expressed in mouse testis.In the testis,distinct immunopositive staining for Atrn protein was detected on cell membrane within spermatogonia,primary spermatocyte,spermatid,Sertoli cell,Leydig cell and peritubular myoid cells.Conclusion：Atrn is expressed in the Leydig cells and germ cells,suggesting that it may play important roles in male reproductive physiological processes.