摘要
目的:从TH1/TH2细胞平衡角度观察清肺口服液对感染RSV肺炎小鼠肺组织内T-bet、GATA3及IL-12的影响,并对其作用机制进行初步探讨。方法:将45只BABL/c小鼠随机分为5组,正常对照组,模型组,利巴韦林组,清肺(3.4 g/mL)组,清肺(10.2 g/mL)组。每组9只,用RSV(Long株)病毒予小鼠滴鼻造模,病毒激发24 h后,分给药72 h后处死小鼠,立即取小鼠肺组织行病理分析,及肺细胞中RT-PCR检测T-bet和GATA3蛋白表达水平,ELISA方法检测肺泡灌洗液(BALF)中IL-12的含量,流式细胞术方法检测小鼠脾脏细胞IL-4和INF-γ表达含量。结果:小鼠模型组与各给药组小鼠肺组织均有T-bet和IL-12(P<0.05,P<0.01)显著升高,GATA3显著降低。结论:清肺口服液具有调节TH1/TH2平衡,并对于感染rsv小鼠体内T-bet及IL-12细胞因子具有明显提升作用,而对GATA3则有明显抑制效果,从而进一步证明了清肺口服液治疗RSV病毒性肺炎有效性。
Objective:To investigate the effects of Qingfei Oral Liquid on expression of T- bet, GATA3 and Interleukin (IL) -12 in mice's lung tissue infected with respiratory syncytial virus(RSV) of disease from the perspective of sustaining Thl/Th2 type cytokines balance. To discuss the mechanism of action. Methods : Forty-five BABIJc mice were classified into five groups ran- domly, nine mice in each group : blank control group, model group, Rihavirin group, Qingfei Oral Liquid low dose group ( 3.4 g/ mL) and high dose group (10.2 g/mL). Mice were molded by RSV (long strain) virus and given 24 -hour's cultivation. 72 hours later they were killed for pathological analysis of lung tissue, RT - PCR test of T'bet and GATA3 protein expression level, ELISA test of expression levels of IL - 12 in BALF and flow cytoinetry test of IL - 4 and INF - ~ expression in the spleen. Results : Both model group and Qingfei Oral Liquid groups showed a significant increase of expressions of T - bet and IL - 12 (P 〈 0. 05, P 〈0. 01 )and a significant decrease of expression of GATA3 in lung tissues. Conclusion:Qingfei Oral Liquid can regulate the bal- ance of Thl/Th2,increase the expressions of T - bet and IL - 12 in the lung tissue of RSV infected mice,inhibit the expression of GATA3,which further proves the effect of Qingfei Oral Liquid in treating the middle stage of RSV induced pneumonia.
出处
《辽宁中医杂志》
CAS
2014年第4期805-807,I0002,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81341086)
江苏省中医院院级课题(Y12049)
科技部重大新药创制项目(2012ZX09303009-002)