HPLC-DAD法同时测定银黄含片中黄芩苷和绿原酸的含量

Determinatina of contents for baicalin and chlorogenic acid in Yinhuang buccal tablet Simultaneously with the method by HPLC-DAD

目的：建立同时测定银黄含片制剂中黄芩苷和绿原酸含量的方法，及测定不同厂家银黄含片中黄芩苷和绿原酸的含量。方法：采用HPLC法，用DAD检测器，岛津VP-ODS C18（250 mm ＆#215;4．6 mm，5（m）色谱柱，流动相：乙腈-0.4％磷酸溶液，梯度洗脱，流速1.0 ml＆#183;min-1，检测波长为绿原酸327 nm、黄芩苷280 nm。结果：绿原酸和黄芩苷分别在0.17～3.40μg和0.33～6.60μg范围内线性关系良好（r＝0.9999），平均加样回收率分别为100.59％（RSD＝0.94％）和98.91％（RSD＝1.14％）。结论：本文建立的方法可以同时准确地测定银黄含片中绿原酸和黄芩苷的含量，方法快速、准确、重复性好，可用于银黄含片的质量控制。

Objective Toestablish the method determinetion on the contents of Baicalin and Chiorogenic acid in the pharmaceutical preparation of Yinhuang Tablet simultaneousiy and to determine the contents of Baicalin and Chlorogenic acid in Yinhuang Tablet of dif-ferent manufacturers.Methods We had adopted the method of HPLC with detector of DAD and chromatogrophy of SHIMADZU VP-ODS C18 （250mmx4.6mm,5μm）,The binder was soiution of acetonitrile -0.4%orthophosphoric acid with gradient elution in the current speed of 1.0ml.min,and the wavelength of detection was 327nm in chlorgenic acid and 280nm in Baicalin.Results There was a good linear relationship diflerently in the range of 0.17-3.40μg in chlorgenic aeid and 0.33μg in Baicalin （r=0.9999）.The Average recov-ery of the added sampie was 100.59% （RSD=0.94%）and 98.91% （RSD=1.14%）Differently.Conclusion The established meth-od in this article could determine accurately the contents of Baicalin and chlorogenic acid in Yinhuang Tablet .And the method was quick,accurate and had a good repeatability,so itcould apply on quality content of Yinhuang Tablet.