唾液中EB病毒抗体检出用于早诊鼻咽癌的研究

Detection of Salivary Epstein-Barr Virus Antibodies for Early Diagnosis of Nasopharyngeal Carcinoma

目的：建立在唾液标本中检出 EB病毒 IgA抗体的方法，以快速、特异诊断鼻咽癌。方法：人工合成 EBV-DNA酶及 EA-D多肽片段，分别作为捕捉抗原，采用 ELISA法检测鼻咽癌患者和健康人血清及唾液中 IgA/ EBV-DNA酶和 EA-D抗体。结果：通过检出 O.D值，经统计学处理，选出鼻咽癌诊断阈值为：血清 O.D值≥ 0.3,唾液 O.D值≥ 0.45（ DNA酶与 EA-D两者阈值一致）。鼻咽癌与健康者两组间 DNA酶与 EA-D的 IgA滴度均为 P< 0.0001,有非常显著性差异，与病理确诊相比，阳性符合率为 72.6％～ 77.3％。结论：证实 EBV合成肽链作为捕捉抗原，唾液为标本，用 ELISA法是可行的，方法简便，重复性好，价廉，但诊断符合率需进一步提高。

Objective： This study was designed to establish a salivary EBV-EA IgA and DNase IgA test technique, and seek a fast and specific diagnostic technique for nasopharyngeal carcinoma (NPC). Methods： Polypeptides of EBV-DNase(ED) and EA-D was synthesized as catching antigens. With ELISA technique, IgA/ED and IgA/EA-D were evaluated respectively in saliva and serum from NPC patients and healthy volunteers. Results： After statistic analysis of the optical density(OD) values of samples, the diagnostic criteria of NPC in the examination of either IgA/ED or IgA/EA-D was defined as following： OD≥ 0.3 for serum and OD≥ 0.45 for saliva. Significantly statistical difference existed between the values of either IgA/ED or IgA/EA-D titer in patients with NPC and the values in healthy volunteers,P< 0.0001. The coincidence rates between the diagnosis of above IgA/ED or IgA/EA-D titer testes and corresponding histological diagnosis were 72.6％－ 77.3％ . Conclusion： The Elisa test to detect salivary IgA/ED and IgA/EA-D with synthesized polypeptides is a simple, repeatable, and cheap technique with stability and sensitivity. However its coincidence rate with histology should be improved.