壬基酚90d染毒对成年SD大鼠胰腺功能的影响

Ninety-Day Nonylphenol Exposure May Cause Adverse Effects on Pancreatic Function in Adult SD Rats

[目的]探讨壬基酚(NP)90 d染毒对大鼠胰腺组织以及血糖相关激素的影响。[方法]雄性成年SD大鼠32只,隔天灌胃染毒90 d,染毒剂量分别为NP 40、100和250 mg/kg体质量。实验期间定时检测体质量、血糖、每日饮食饮水量。染毒后测血清中胰岛素、己糖激酶以及游离脂肪酸含量;胰腺组织称重并做病理组织学检查;用TUNEL法检测胰腺细胞凋亡程度;计算胰腺脏器系数、胰岛素敏感指数、胰岛素分泌指数。[结果]与对照组比较,NP染毒组大鼠胰腺脏器系数降低(P<0.01),日均饮水量增加(P<0.01),血清胰岛素(P<0.05)、己糖激酶降低(P<0.01),胰岛素敏感指数增高(P<0.01);胰腺胰岛数量及大小均减小,胰腺细胞凋亡无明显变化。[结论]NP亚慢性暴露可损伤大鼠胰腺功能,并对其相关激素产生一定影响。

[E Objective ] To examine the effects of exposure to nonylphenol （NP） for 90 days on pancreas and relevant indicators of blood glucose in rats. [ Methods ] Thirty-two adult male Sprague-Dawley （SD） rats were randomly divided into four groups, fed with NP by gavage at doses of 0, 40, 100, or 250mg/kg body weight on alternate days for a period of 90days respectively. Body weight, daily food and water intake, and blood glucose were measured regularly. After last gavage, insulin, hexokinase （HK）, free fatty acids （FFA） in the serum were detected. Hematoxylin-eosin （H＆E） staining method and TdT-mediated dUTP nick end labeling （TUNEL） technique were used to analyze histological changes and cell apoptosis of pancreas. Pancreas coefficient, insulin sensitive index, and insulin secretion index were also calculated. [ Results ] Compared with the control group, the coefficient of pancreas （P〈 0.01）, serum insulin （P 〈 0.05）, and serum HK were significantly decreased （P 〈 0.01）, while daily water intake and insulin sensitive index were increased （P〈 0.01） in the NP-administrated groups （40, 100, and 250 mg/kg）. The size and number of islets were reduced. However, no apoptosis was found in the pancreatic cells. [ Conclusion ] It may speculate that 90-day exposure of NP poses some adverse effects on pancreatic function and has an impact on relevant indicators.