PB231诱导K562细胞凋亡的初步研究

Primary study on apoptosis in K562 cells induced by PB231

目的 研究PB2 3 1诱导K 562细胞凋亡作用 ,探讨PB2 3 1的抗肿瘤机制。方法 采用荧光显微镜观察细胞形态学变化 ,MTT法测定PB2 3 1对K 562细胞生长抑制作用 ,琼脂糖凝胶电泳检测细胞DNA裂解。结果 K562细胞经PB2 3 1处理后 ,在倒置光显微镜下可见细胞变形和凋亡小体形成 ;荧光显微镜下可见染色质凝集。MTT法检测其IC50 值为 5× 1 0 -5M。琼脂糖凝胶电泳呈典型的“DNALadder”。结论 PB2 3 1可诱导K562细胞发生凋亡。这可能是其抑制K562细胞生长的作用机理之一。

Objective TO study whether PB231 can induce apoptosis of K562 cell and its mechanism of antitumor.Methods Using experiment in vitro;we added PB231 of different concentrations into the medium in which the cells were cultured.The apoptosis was detected by fluorescence microscope and gel electrophoresis.Cytoxity rate was detected by MTT.Results The cell growth was remarkably inhibited by PB231,the IC 50 volues of PB231 for K562 cells were found to be.5×10 -5 M;the typical nuclear condensation and fragmentation were observed by fluorescence microscope;“DNA ladder”appeard on a 1.5% agarose gel.Conclusions Induction of apoptosis contributes to the mechanisms for antitumor activity of PB231.