p53、Rb、IGF-I、AT_(1B)基因转移对血管新生内膜增殖的影响

Effects of human wild-type p53, Rb, antisense human IGF-I and antisense rat AT_(1B) gene transfer on neointimal proliferation after carotid artery injury in rat

目的观察和比较人野生型ｐ５３基因、Ｒｂ基因、反义ＩＣＦ－Ｉ基因与大鼠反义ＡＴ１Ｎ基因对大鼠颈动脉损伤后新生 内膜增殖的影响。方法通过病毒载体介导，将上述４种基因分别转导至大鼠球囊损伤的颈动脉再狭窄模型中，２１ｄ后 观察并比较其对新生内膜形成的影响。结果与对照组相比，ｐ５３基因、Ｒｂ基因治疗组及反义ＩＧＦ－Ｉ基因治疗组的动脉 血管新生内膜／中层面积比均显著降低（ｐ＜０．０１）；与ＡｄＶβ ｇａｌ组相比，反义ＡｄＶＡＴ１Ｂ基因组的动脉血管新生内膜／中 层面积比显著降低（Ｐ＜０．０１）．而ｐ５３基因治疗组、Ｒｂ基因治疗组、反义ＩＧＦ－Ｉ基因组及反义ＡｄＶＡＴ１Ｂ基因组之间无显 著性差异（Ｐ＜０．０５）。结论上述４种基因对血管成形术后再狭窄可能均有一定的预防作用。

Objective To make a comparative study of the effects of human wild-type p53, Rb, antisense human IGF-I and antisense rat A T1B. gene transfer on neointimal proliferation following caroid arterial injury in rats. Methods Rat models of carotid artery restenosis was established by balloon in injury and transduction of each of the above 4 genes into the injured rat unilateral carotid arteries via viral vector LSXN was respectively performed. The rats subjected to either LSXN or AdVβ gal treat- ment following the injury served as control groups. Neointima/media ratio (N/MR) at the site of arterial injury was determined 21 d after balloon in injury. Results Compared with the control groups, N/NR in rats with human wild-type p53, Rb, antisense human ICF-I and antisense rat A T1B. gene transfer showed significant reduction by 44%, 49%, 41% and 47% respectively (P<0.01). Conclusion The 4 genes may provide partial protection against restenosis after angioplasty.