VitE及oxLDL对人单核细胞表达CD40和CD40L的影响

Effects of oxidized low density lipoprotein and antioxidant vitamin E on the expression of CD40 and CD40 ligand in cultured human monocytes

目的 :探讨氧化低密度脂蛋白 (ox L DL )和抗氧化剂 Vit E对人单核细胞表达 CD40和 CD40配体 (D40 L )的影响。 方法 :应用流式细胞术间接免疫荧光法检测细胞表面 CD40和 CD40 L表达水平。结果 :低浓度 ox L DL (≤ 2 0 0μg/ L )可使单核细胞表达 CD40和 CD40 L明显增加 ,具有浓度和时间依赖效应。高浓度 (>2 0 0μg/ L ) ox L DL刺激时 ,CD40和 CD40 L表达明显下降。预先加入抗氧化剂 Vit E后 ,低浓度 ox L DL 刺激单核细胞表达 CD40和 CD40 L 明显下降 ,并呈浓度依赖效应。结论 :ox L DL 刺激单核细胞高表达 CD40和 CD40 L 可能是其致动脉粥样硬化的重要机制之一。抗氧化剂 Vit E能部分抑制 ox L DL引起的单核细胞高表达 CD40和 CD40

Objective: To investigate the effect of oxLDL and VitE on the expression of CD40 and CD40 ligand(CD40L) in cultured human monocytes. Methods: The expression of CD40 and CD40L on monocytes surface were measured by indirect immunorescence technique in combination with flow cytometry. Results: Low concentration of oxLDL(≤200 μg/L) significantly increased the expression of CD40 and CD40L in a dose and time dependent manner. High concentration (>200 μg/L)of oxLDL markedly reduced the expression of CD40 and CD40L. When VitE was added, it significantly reduced the expression of CD40 and CD40L on monocytes surface induced by oxLDL in a dose dependent manner. Conclusion:It is an important mechanism that the high expression of CD40 and CD40L induced by oxLDL may be contributed to the formation of atherosclerosis. Antioxidan VitE can partially inhibit the high expression of CD40 and CD40L on monocytes surface induced by oxLDL. [