摘要
目的探讨骨形态发生蛋白-7（BMP-7）对大鼠脑缺血再灌注（I-R）氧化应激损伤的影响。方法成年雄性Wistar大鼠60只，随机分为假手术组、I-R组、BMP-7组，每组20只，采用线栓法制作大脑中动脉I-R模型，观察BMP-7对大鼠神经功能缺损评分、脑梗死体积、丙二醛（MDA）含量及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH—PX）活性的影响。结果与Ht组相比，BMP-7组大鼠神经功能缺损评分降低（t=2．33，P〈0．05），脑梗死体积减小（t=5．51，P〈0．05），MDA含量降低（F活性升高（F=22．19±68．11，q=9．41～16．25，P〈0．05）。能与清除自由基，减轻氧化性损伤有关。33．99、42．94，q=6．59、6．16，P〈0．05），SOD和GSH—PX结论BMP-7对大鼠I-R损伤具有保护作用。
Objective To investigate the protective effect of bone morphogenetic protein-7 （BMP-7） on focal cerebral ischemia reperfusion （I-R） injury in rats. Methods Sixty adult male Wistar rats were equally randomized to three groups as： sham-operated group, I-R group, and BMP-7 group. A model of I-R was created in rats by a middle cerebral artery occlusion. The effect of BMP-7 on neurological deficits, infarction volume of brain, content of malondialdehyde （MDA） , activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-PX） were observed. Results Compared with the I-R group, neurological deficits score in BMP-7 group decreased （t = 2.33, P〈0.05）, infarction volume of brain reduced （t = 5.51, P 〈0.05）, the content of MDA declined （F=33.99,42.94;q=6.59,6.16;P〈0.05）. the active of SOD and GPX-PX improved （F=22.19-68.11,q= 9.41-16.25,P〈0.05）. Conclusion BMP-7 has a protective effect on cerebral ischemia reperfusion injury by reducing the free radicals and oxidative damage.
出处
《青岛大学医学院学报》
CAS
2014年第2期141-143,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
青岛市科技局科研基金资助项目(09-1-3-78-JCH)
