鼻咽癌组织端粒酶各组分基因表达的研究

Expression of Telomerase Subunits in Nasopharyngeal Carcinoma

目的：探讨端粒酶各组分基因在鼻咽癌（ nasopharyngeal carcinoma,NPC）组织及鼻咽慢性炎症粘膜（ chronic inflammation of nasopharyngeal epithelium,CINE）组织中的表达情况。方法：利用 RT-PCR方法检测鼻咽癌组织及鼻咽慢性炎症粘膜组织端粒酶各组分基因（ hTR、 TP1 mRNA和 hTERT mRNA）的表达。结果： 43例 NPC组织中， hTR、 TP1 mRNA和 hTERT mRNA表达阳性率分别为 90.7％、 88.4％和 88.4％； 16例 CINE组织中 ,hTR、 TP1 mRNA和 hTERT mRNA表达阳性率为 87.5％、 87.5％和 0％；仅 hTERT mRNA在鼻咽癌组织中的表达显著高于鼻咽慢性炎症粘膜组织中的表达，而 hTR或 TP1 mRNA在鼻咽癌组织中的表达和鼻咽慢性炎症粘膜组织中的表达无明显差异。表明 hTR和 TP1 mRNA广泛存在于鼻咽癌和鼻咽慢性炎症粘膜组织中，而 hTERT mRNA仅在鼻咽癌组织中表达。结论： hTERT mRNA可能在端粒酶活性调节中起重要作用； hTERT mRNA的表达水平，可作为鼻咽癌诊断指标之一。

Objective： This study was designed to ascertain telomerase subunits expression in nasopharyngeal carcinoma (NPC) as well as in chronic inflammation of nasopharyngeal epithelium (CINE). Methods： Telomerase subunits(hTR,TP1 mRNA, hTERT mRNA) expression was determined with reverse transcription-polymerase chain reaction (RT-PCR) in 43 cases of NPC and 16 cases of CINE. Results： The positive rates of hTR,TP1 mRNA and hTERT mRNA were 90.7％ ,88％ , and 88％ in 43 NPCs, and 87.5％ , 87.5％ , and 0 in 16 CINEs respectively. hTERT mRNA exoressuib was significantly higher in NPC than in CINE,but no statistical difference for hTR or TP1 mRNA occurred in two groups of patients. Conclusion： These findings revealed that hTR and TP1 mRNA were widely expressed in both NPC and CINE, but hTERT mRNA was limited in NPC, which suggests that hTERT mRNA may play a critical role in activation of telomerase. The authers findings also indicate that detection of hTERT mRNA may be a useful maker for clinical diagnosis of NPC.