摘要
采用高效液相-四级杆飞行时间质谱联用技术(HPLC-QTOF/MS)建立了冬虫夏草高效液相特征图谱。色谱条件为Agilent Zorbax SB Aq(150 mm×4.6 mm,5μm)色谱柱;流动相为5 mmol·L-1乙酸铵和乙腈,梯度洗脱;流速0.8 mL·min-1;检测波长260 nm。该方法分别从冬虫夏草中鉴定了9个化学成分,冬虫夏草无性型中华被毛孢菌丝体中鉴定了9个化学成分,北冬虫夏草中鉴定了8个化学成分,成功运用于3种虫草样品分析。
A HPLC-QTOF MS method was established for analysis of components in Ophiocordyceps sinensis . The HPLC analysis was performed on an Agilent Zorbax SB Aq （150 mm×4.6 mm, 5 μm） with gr adient elution （5 mmol·L-1 ammonium acetate aqueous solution-acetonitrile）, flow rate was 0.8 mL·min-1 and detection wave-length was 260 nm. The developed method was successfully applied in analysis of three different samples in-cluding O. sinensis, Hirsutella sinensis （ anamorph of O.sinensis） and Cordyceps militaris. Nine compounds were i-dentified in both O.sinensis and H.sinensis, which eight compounds were identified in C.militaris.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第2期279-283,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
湖北省科学技术厅重大科技创新计划项目(ZDS0042):仿生态培植冬虫夏草成果转化
负责人:李文佳
