长沙地区母婴HCMV感染的分子流行病学初步研究

Study on molecular epidemiology of HCMV infection in mothers and their newborns in Changsha

利用ELISA及PCR技术对长沙地区 12 5对母、婴HCMV IgG ,IgM及HCMV DNA进行了检测。结果显示 :在 12 5对母、婴中 ,HCMV IgG阳性率分别为 95 .2 % (119/ 12 5 )和 93.6 % (117/ 12 5 ) ,HCMV IgM阳性率分别为 5 .6 % (7/12 5 )和 0 .8% (1/ 12 5 ) ,血浆HCMVDNA阳性率分别为 11.2 % (14/ 12 5 )和 5 .6 % (7/ 12 5 ) ,P(C)BMCsHCMVDNA阳性率分别为 2 1.6 % (2 7/ 12 5 )和 14.4% (18/ 12 5 )。新生儿CBMCS HCMVDNA阳性率显著高于HCMV IgM及血浆HCMVDNA的阳性率 (P <0 .0 5 ) ,而血浆PCR与IgM阳性率差异无显著性意义 (P >0 .0 5 )。新生儿CBMCSHCMVDNA用于判断其先天性感染的灵敏度为 81.8%。表明CBMCSHCMVDNA与血浆HCMV IgG分别为新生儿及产妇HCMV感染的较好的流行病学指标。

The detection of HCMV IgG,IgM in plasma by ELISA and DNA in plasma and P(C)BMCs from mothers and their newborns by PCR was carried out. Positive HCMV IgG, HCMV IgM,P(C)BMCs HCMV DNA and plasma HCMV DNA were demonstrated in 119(95.2%),7(5.6%),27(21.6%)and 14(11.2%) in 125 mothers and 117(93.6%), 1(0.8%),18(14.4%) and 7(5.6%) in their mewborns, respectively. There were significant differences among the positive rate of newborn's HCMV IgM, plasma HCMV DNA and CBMCs HCMV DNA (P<0.05),but the difference between HCMV IgM and plasma HCMV DNA had no statistic significance(P>0.05). This study suggests that HCMV IgG and CBMCs HCMV PCR are preferable epidemiological index of HCMV infection of pregnant women and newborns respectively. [