人精子穿透去透明带金黄地鼠卵试验与人精卵体外受精的比较

Comparison Between Human Sperm Zona free Hamster Ovum Penetratio n Assay and In vitro Fertilization

目的 研究在人工助孕技术中能否以人精子穿透去透明带金黄地鼠卵试验 ( SPA)结果作为确定采用人精卵体外受精 ( IVF)或经卵细胞胞浆内单精子注射 ( ICSI)的指标。方法 对 2 2例准备行 IVF的夫妇男方精子 SPA受精率与其 IVF受精率结果进行比较。结果 SPA和 IVF受精率分别为 38.6 %± 2 3.5 %与 5 3.5 %±2 8.4% ,SPA受精率较 IVF受精率低 ,但随着 SPA受精率的增加 ,IVF受精率也呈明显增加的趋势。在 SPA受精率大于 10 %的病例中 ,约 76 %的病例 IVF受精率大于 5 0 %。经统计学分析两者有显著相关性 ( rs=0 .70 46 ,| U | =3.2 2 84>u0 .0 1 /2 )。结论 SPA受精率可作为助孕技术中确定 IVF或

Objective The research question was whether the r esult of human sperm zona free hamster ovum penetration assay(SPA) could serve as an indicator for in vitro fertilization (IVF) or intracytoplasmic sperm inje ction(ICSI).Methods The semen samples of 22 male partners in c ouples with infertile marriage were assessed for their fertilizing capac ity by SPA. The fertilization rate of SPA was compared with that of IVF. Results The fertilization rates of SPA and IVF were 38 6%±23 5% an d 53 5%±28 4% respectively, and a significant correlation between SPA and IVF was observed (rs= 0.7045). Conclusion The result of SPA may be used as a referenc e indicator for assisting fertilization,IVF or ICSI.