顺铂诱导原代培养人膀胱癌细胞的凋亡

Apoptosis induced by cisplatin in primary culture bladder cancer cells

目的 :探讨顺铂诱导原代培养膀胱癌细胞发生调亡变化的特征及机制。方法 :以 0— 10 0 μmol L浓度顺铂处理原代培养膀胱癌细胞 12— 72小时 ,应用MTT法检测细胞增殖抑制变化 ,分析其与凋亡指数 (AI)的关系 ;用光镜和透射电镜分别观察膀胱癌细胞凋亡变化的特征。结果 :顺铂能够明显地诱导膀胱癌细胞凋亡 ,具有一定范围内的作用时间和药物浓度依赖性 ,与细胞增殖抑制密切相关。膀胱癌细胞凋亡的镜下特征为胞膜完整 ,胞浆浓缩 ,核碎裂、萎缩或边积 ,胞浆外突形成凋亡小体。结论

Purpose:To investigate the mechanism of characteristic morphological changes of primary culture bladder cancer cells in vitro in the process of apoptosis induced by cisplatin (DDP). Methods:From 12—72 hours primary bladder cancer cells were treated by CRM (complete RPMI1640 medium) which contained 0—100 μmol/L DDP, the proliferation of the tumor cells inhibitied by DDP was determined by MTT assay, and its relationship with AI (apoptotic index)was evaluated. In addition, apoptotic feature of tumor cells death induced by DDP was observed by using a light microscope and a transmission electron microscope.Results:An extensive apoptosis in primary culture bladder cancer cells treated by DDP occurred, which was dependent on an appropriate concentration of the drug or treatment times, and was associated with the proliferation of the cells inhibited by DDP. The observation showed apoptosis of the cells is characterized by protuberances of normal cell membrane and condensation of cytoplasm containing a few round vacuoles that separate from the cell surface to produce apoptotic bodies, following the nucleus fragments, and compaction and migration of nuclear chromatin. Conclusions:An extensive apoptosis induced by DDP in the primary culture bladder cancer cells is involved in the process of bladder cancer cells killed by DDP.