球孢白僵菌菌株交配型分子鉴定

Molecular Identification of Strain Mating Type of Beauveria Bassiana

球孢白僵菌Beauveria bassiana是一种重要的虫生真菌,开发的真菌杀虫剂是一类优良的生物农药,其有性世代—球孢虫草Cordyceps bassiana也有潜在的经济价值.鉴定球孢白僵菌菌株的交配型,确立含有不同交配型标准菌株,进行菌株的快速配对,对于球孢虫草的人工栽培和球孢白僵菌的育种都具有重要的价值.通过多次重复试验,优化了扩增条件,保证了检测结果的稳定性和可靠性.该试验结果为检测球孢白僵菌菌株的交配型提供了一种简便、准确的方法,并为研究球孢白僵菌交配型基因的功能、人工栽培和育种奠定了基础.

Beauveria bassiana is an important fungal species. Development of fungal pesticide is an excellent class of biological pesticides. Their sexual generation Cordyceps bassiana has potential economic value. Identification of B. bassiana strain mating type,establishment standard strains containing different mating types,Pairing strains for rapid are of great value to artificial cultivation and breeding of Cordyceps bassiana for B. bassiana. By repeated tests,the amplification conditions are optimized. and the stability and reliability of test results are ensured. The test results for the detection of B. bassiana strain mating type,a simple and accurate method is provided. And for the study of B. bassiana mating type gene function, artificial cultivation and breeding,the foundation is established.