摘要
将狂犬病病毒核蛋白(N)与麦芽糖结合蛋白(MBP)在大肠杆菌中融合表达。采用Western blot和酶联免疫吸附试验(ELISA)检测该重组融合蛋白的抗原性,并建立了间接ELISA方法检测狂犬病病毒特异性抗体。ELISA结果与快速荧光抑制试验(RFFIT)检测的中和抗体进行比较,RFFIT检测的中和效价与ELISA检测的OD值相关性很好(r=0.943 6),并且ELISA的敏感性和特异性分别为93.4%和100%。
The nucleoprotein （N） gene of rabies virus was expressed in Escherichia coli as a fusion with maltose binding protein （MBP）. The antigenicity of this recombinant MBP- N fusion protein was examined by Western blot and enzyme linked immunosorbent assay （ELISA）. An indirect ELISA was developed to detect specific antibody against rabies virus. The ELISA results were compared with virus neutralizing antibodies detected by a rapid fluorescent focus inhibition test （RFFIT）. Neutralizing titres by RFFIT were found to correlate well with the OD values in the established ELISA （ r=0. 943 6） and the sensitivity and specificity of the ELISA were shown to be 93.4% and 100%, respectively.
出处
《畜牧与兽医》
北大核心
2014年第4期30-35,共6页
Animal Husbandry & Veterinary Medicine
基金
河北省教育厅支持项目(Z2007212)
