胃腺癌的血管内皮细胞生长因子和KDR受体的表达及其临床意义

Expression of Vascular Endothelial Cell Growth Factor and KDR Receptor in Gastric Adenocarcinoma and its Clinical Significance

目的：研究胃腺癌的血管内皮细胞生长因子（ｖａｓｃｕｌａｒ ｅｎｄｏｔｈｅｌｉａｌ ｃｅｌｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ， ＶＥＧＦ）及其受体 ＫＤＲ（ＶＥＧＦＲ）的表达，并探讨其临床意义。方法：应用免疫组织化学染色法检测ＶＥＧＦ／ＶＥＧＦＲ的表达，并分析其与６１例胃腺癌临床病理特征之间的关系。结果：胃腺癌组织ＶＥＧＦ／ＶＥＧＦＲ的阳性表达率分别为５５．９％和３９．３％；其中粘液腺癌和管状腺癌表达率较高（Ｐ＜０．０５，Ｐ＜０．０１），ＶＥＧＦ／ＶＥＧＦＲ表达率分别为７１．４Ｔ／４２．８％和６１．９％／５２．４％；ＶＥＧＦ／ＶＥＧＦＲ的阳性表达与ＴＮＭ分期有显著差异（Ｐ＜０．０５，Ｐ＜０．０１），阳性率为Ⅵ＞Ⅲ＞Ⅱ＞Ⅰ期，Ⅳ、Ⅲ、Ⅱ、Ⅰ期的表达率分别为 ８０．０％／５０．０％，６８．２％／４５．５％，４０．０％／３０．０％和３６．８％／３１． ６％；胃腺癌并转移患者，ＶＥＧＦ／ＶＥＧＦＲ的阳性表达分别为７６．２％和４７．６％，远高于无转移的４５．０％和３５．０％（Ｐ＜０．０５，Ｐ＜０．０１）；术后生存期小于１年的ＶＥＧＦ的表达率为６６．７％，明显高于术后生存期１年以上的５２．２％。

Objective: The current study was designed to investigate the expression of vascular endothelial cell growth factor (VEGF) and its receptor KDR (VEGFR) in gastric adenocarcinoma and its clinicopathologic significance. Methods: Expression of VEGF/VEGFR in gastric adenocarcinoma was determined by immunohistochemical staining, and the relationship between VEGF/VEGFR expression and the pathological features in 61 cases of gastric adenocarcinoma were analyzed. Results: The positive VEGF/VEGFR expression were detected in 34(55. 9%) and 24 (39. 3% ) tumor samples respectively. The expressions of VEGF/VEGFR in mucous adenocaroinoma(71. 4% /42. 8% ) and in tubular adenocarcinoma (61. 9% /52.4%) were obviously higher than those of the other three classes of gastric adenocaroinoma(P<0. 05, P< 0. 01 ). VEGF/VEGFR expression was significantly related to pathological TNM grade(P <0. 05, P < 0. 01 ), and the positive rates of Grade Ⅳ, Ⅲ, Ⅱ and Ⅰ were 80. 0% /50. 0%, 68. 2% /45. 5%, 40. 0% /30. 0% and 36. 8% /31. 6%, respectively. A strong correlation was found between VEGF/VEGFR expression and gastric adenocarcinoma metastasis(P < 0. 05, P<0. 01). With regard to prognosis, VEGF positive tumors(66. 7% ) of under one-year survival was significantly higher than that of over one-year survival(52. 2% ). Conclusions: VEGF is a promote factor responsible for the induction of angiogenesis in gastric adenocarcinoma. VEGF/VEGFR positive expression may be a useful indicator for estimation of metastasis, pathological staging and prognosis in gastric adenocarcinoma.