妊娠高血压综合征患者胎盘组织中血管内皮生长因子的表达

Expression of vascular endothelial growth factor in placenta from pregnancy complicated with pregnancy induced hypertention

目的 探讨血管内皮生长因子 (VEGF)在妊娠高血压综合征 (妊高征 )患者胎盘组织中的表达及对妊高征患者胎盘滋养细胞功能和绒毛毛细血管网形成的影响。方法 采用免疫组织化学SP法检测 2 1例正常孕妇 (对照组 ) ,6 0例妊高征患者 (妊高征组 ,其中轻、中、重度妊高征患者各 2 0例 )胎盘组织VEGF的表达强度。结果 VEGF主要表达于胎盘绒毛滋养细胞 ;中度及重度妊高征患者胎盘绒毛滋养细胞VEGF表达强度均明显低于对照组及轻度妊高征患者 (P <0 0 5 ) ,而轻度妊高征患者与对照组比较 ,差异无显著性 (P >0 0 5 ) ,各组间蜕膜组织VEGF表达强度差异无显著性 (P>0 0 5 )。 结论 VEGF在胎盘中主要由绒毛滋养细胞分泌 ,妊高征患者VEGF分泌减少 ,可能是引起局部胎盘绒毛及血管病变的原因之一。

Objective To study the expression of vascular endothelial growth factor (VEGF) in placenta from normal pregnancy and pregnancy complicated with pregnancy induced hypertension (PIH) and the effect of VEGF on the etiology of PIH. Method Use the immunohistochemistry to determine the location and intensity of VEGF staining in placenta from 21 cases of normal pregnancy and 62 cases of mild, moderate and severe group of pregnancies complicated with PIH. Result Immunolocalization of VEGF in all cases was observed mainly in syncytiotrophoblast. Intensity of VEGF immnostaining in syncytiotrophoblast was significantly reduced in the moderate PIH group ( P <0 05) and the severe PIH group ( P <0 01) compared with both the mild PIH group and the normal group, while there was no qualitative differences between the mild PIH group and the normal group. Intensity of staining in decidual cells was also similar in all the groups. Conclusion These results suggest that VEGF is secreted mainly by syncytiotrophoblast in human placenta and the reduced VEGF may be responsible, at least in part, for the impaired vascular development in PIH.